Human Caspase 1(Casp-1) ELISA Kit CSB-E13025h CUSABIO
$50.00
In stock
SKU
CSB-E13025h
Product Details
| Uniprot No. | P29466 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.39 ng/ml |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | CASP-1 ELISA Kit; CASP1 ELISA Kit; CASP1_HUMAN ELISA Kit; Caspase 1 ELISA Kit; Caspase-1 subunit p10 ELISA Kit; ICE ELISA Kit; IL-1 beta-converting enzyme ELISA Kit; IL-1BC ELISA Kit; IL1 beta converting enzyme ELISA Kit; IL1B convertase ELISA Kit; Interleukin 1 beta convertase ELISA Kit; Interleukin 1B converting enzyme ELISA Kit; Interleukin-1 beta convertase ELISA Kit; Interleukin-1 beta-converting enzyme ELISA Kit; p45 ELISA Kit |
| Abbreviation | CASP1 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 1.56 ng/ml - 100 ng/ml |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cell Biology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human Casp-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human Casp-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human Casp-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 97; 1: Range %; 2: 92-103; 1: 1:2; 2: Average %; 3: 104; 1: Range %; 2: 99-109; 1: 1:4; 2: Average %; 3: 101; 1: Range %; 2: 94-108; 1: 1:8; 2: Average %; 3: 93; 1: Range %; 2: 89-98 |
| Recovery | headers: The recovery of human Casp-1spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human Casp-1spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human Casp-1spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 102; 3: 97-108; 1: EDTA plasma (n=4); 2: 99; 3: 92-105 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 100; 2: 2.644; 3: 2.634; 4: 2.639; 5: 2.448; 1: 50; 2: 2.082; 3: 2.067; 4: 2.074; 5: 1.884; 1: 25; 2: 1.470; 3: 1.428; 4: 1.449; 5: 1.258; 1: 12.5; 2: 0.955; 3: 0.929; 4: 0.942; 5: 0.751; 1: 6.25; 2: 0.547; 3: 0.521; 4: 0.534; 5: 0.343; 1: 3.12; 2: 0.451; 3: 0.453; 4: 0.452; 5: 0.261; 1: 1.56; 2: 0.325; 3: 0.314; 4: 0.319; 5: 0.129; 1: 0; 2: 0.186; 3: 0.195; 4: 0.191 |
| Materials provided | A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human Casp-1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates. Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. One vial Biotin-labeled Casp-1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody. One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic. One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody. One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution. One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration. One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances. One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation. An instruction manual |
| Materials not provided | A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. An incubator can provide stable incubation conditions up to 37°C±5°C. Centrifuge Vortex Squirt bottle, manifold dispenser, or automated microplate washer Absorbent paper for blotting the microtiter plate 50-300ul multi-channel micropipette Pipette tips Single-channel micropipette with different ranges 100ml and 500ml graduated cylinders Deionized or distilled water Timer Test tubes for dilution |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | The Human Caspase 1 (Casp-1) ELISA Kit is suitable for quantitative detection of Casp-1 in serum, plasma, and tissue homogenates. Samples or standards are added into the microwells and bind to the anti-human Casp-1 antibody immobilized on the microplate. A sandwich is formed by the addition of the biotin-conjugated Casp-1 antibody. The TMB substrate solution is added that reacts with the enzyme-antibody-target complex to elicit a chromogenic reaction. The intensity of the solution color is directly proportional to the concentration of Casp-1 present in the original specimen. This kit has been verified with a wide detection range, high sensitivity, excellent specificity, precision less than 10%, good linearity, high recovery, and good consistency between lots. Refer to the product instructions for more verification details. Casp-1 is synthesized as an inactive zymogen that is recruited and activated by inflammasomes. It plays a crucial role in cell immunity as an inflammatory response inhibitor. After activation by inflammasomes, Casp-1 initiates a pro-inflammatory response through the cleavage and subsequent activation of the two inflammatory cytokines, IL-1β and IL-18 as well as gasdermin D (GSDMD)-mediated pyroptosis, thus killing the infectious agent. In the absence of GSDMD, Casp-1 induces apoptosis involves the Bid-caspase-9-caspase-3 axis, which can elicit secondary necrosis/pyroptosis mediated by GSDME/DFNA5. |
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