Human Caspase 8(Casp-8) ELISA Kit CSB-E13636h CUSABIO
$50.00
In stock
SKU
CSB-E13636h
Product Details
| Target Name | caspase 8, apoptosis-related cysteine peptidase |
| Uniprot No. | Q14790 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 7.81 pg/ml |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | ALPS2B ELISA Kit; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein ELISA Kit; Apoptosis related cysteine peptidase ELISA Kit; Apoptotic cysteine protease ELISA Kit; Apoptotic protease Mch-5 ELISA Kit; Apoptotic protease Mch5 ELISA Kit; CAP 4 ELISA Kit; CAP4 ELISA Kit; CASP-8 ELISA Kit; CASP8 ELISA Kit; CASP8_HUMAN ELISA Kit; Caspase 8 ELISA Kit; Caspase 8 apoptosis related cysteine peptidase ELISA Kit; Caspase IIX ELISA Kit; Caspase-8 subunit p10 ELISA Kit; caspase8 ELISA Kit; CED 3 ELISA Kit; FADD Homologous ICE/CED3 Like Protease ELISA Kit; FADD Like ICE ELISA Kit; FADD-homologous ICE/CED-3-like protease ELISA Kit; FADD-like ICE ELISA Kit; FLICE ELISA Kit; FLJ17672 ELISA Kit; ICE-like apoptotic protease 5 ELISA Kit; MACH alpha 1/2/3 protein ELISA Kit; MACH ELISA Kit; MACH beta 1/2/3/4 protein ELISA Kit; MACH5 ELISA Kit; MCH 5 ELISA Kit; MCH5 ELISA Kit; MGC78473 ELISA Kit; MORT1 associated ced 3 homolog ELISA Kit; MORT1 associated CED3 homolog ELISA Kit; MORT1-associated CED-3 homolog ELISA Kit; OTTHUMP00000163717 ELISA Kit; OTTHUMP00000163720 ELISA Kit; OTTHUMP00000163724 ELISA Kit; OTTHUMP00000163725 ELISA Kit; OTTHUMP00000165062 ELISA Kit; OTTHUMP00000165063 ELISA Kit; OTTHUMP00000165064 ELISA Kit; OTTHUMP00000206552 ELISA Kit; OTTHUMP00000206582 ELISA Kit |
| Abbreviation | CASP8 |
| Sample Types | serum, plasma, cell lysates |
| Detection Range | 31.25 pg/mL-2000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cell Biology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human CASP-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human CASP-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human CASP-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 86; 1: Range %; 2: 80-96; 1: 1:2; 2: Average %; 3: 90; 1: Range %; 2: 84-102; 1: 1:4; 2: Average %; 3: 95; 1: Range %; 2: 92-100; 1: 1:8; 2: Average %; 3: 93; 1: Range %; 2: 83-99 |
| Recovery | headers: The recovery of human CASP-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human CASP-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human CASP-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 95; 3: 87-103; 1: EDTA plasma (n=4); 2: 97; 3: 90-105; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 2000 2.226 2.238 2.232 2.136 1000 1.513 1.546 1.530 1.434 500 0.917 0.932 0.925 0.829 250 0.588 0.579 0.584 0.488 125 0.406 0.394 0.400 0.304 62.5 0.251 0.237 0.244 0.148 31.25 0.139 0.133 0.136 0.040 0 0.095 0.097 0.096; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 2000; 9: 2.226; 10: 2.238; 11: 2.232; 12: 2.136; 14: 1000; 15: 1.513; 16: 1.546; 17: 1.530; 18: 1.434; 20: 500; 21: 0.917; 22: 0.932; 23: 0.925; 24: 0.829; 26: 250; 27: 0.588; 28: 0.579; 29: 0.584; 30: 0.488; 32: 125; 33: 0.406; 34: 0.394; 35: 0.400; 36: 0.304; 38: 62.5; 39: 0.251; 40: 0.237; 41: 0.244; 42: 0.148; 44: 31.25; 45: 0.139; 46: 0.133; 47: 0.136; 48: 0.040; 50: 0; 51: 0.095; 52: 0.097; 53: 0.096; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 2000; 2: 2.226; 3: 2.238; 4: 2.232; 5: 2.136; 1: 1000; 2: 1.513; 3: 1.546; 4: 1.530; 5: 1.434; 1: 500; 2: 0.917; 3: 0.932; 4: 0.925; 5: 0.829; 1: 250; 2: 0.588; 3: 0.579; 4: 0.584; 5: 0.488; 1: 125; 2: 0.406; 3: 0.394; 4: 0.400; 5: 0.304; 1: 62.5; 2: 0.251; 3: 0.237; 4: 0.244; 5: 0.148; 1: 31.25; 2: 0.139; 3: 0.133; 4: 0.136; 5: 0.040; 1: 0; 2: 0.095; 3: 0.097; 4: 0.096 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human CASP8 ELISA Kit was designed for the quantitative measurement of Human CASP8 protein in serum, plasma, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/ml. |
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