Human Cluster of differentiation 8,CD8 ELISA Kit CSB-E12707h CUSABIO
$50.00
In stock
SKU
CSB-E12707h
Product Details
| Target Name | CD8a molecule |
| Uniprot No. | P01732 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 15.6 U/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | alpha polypeptide (p32) ELISA Kit; CD8 ELISA Kit; CD8 antigen alpha polypeptide ELISA Kit; CD8 antigen alpha polypeptide (p32) ELISA Kit; CD8a ELISA Kit; CD8A antigen ELISA Kit; CD8A molecule ELISA Kit; CD8A_HUMAN ELISA Kit; Leu2 ELISA Kit; Leu2 T lymphocyte antigen ELISA Kit; Ly3 ELISA Kit; LYT3 ELISA Kit; MAL ELISA Kit; OKT8 T cell antigen ELISA Kit; OTTHUMP00000160760 ELISA Kit; OTTHUMP00000160764 ELISA Kit; OTTHUMP00000203528 ELISA Kit; OTTHUMP00000203721 ELISA Kit; p32 ELISA Kit; T cell antigen Leu2 ELISA Kit; T cell co receptor ELISA Kit; T-cell surface glycoprotein CD8 alpha chain ELISA Kit; T-lymphocyte differentiation antigen T8/Leu-2 ELISA Kit; T8 T cell antigen ELISA Kit; T8/Leu-2 T-lymphocyte differentiation antigen ELISA Kit |
| Abbreviation | CD8A |
| Sample Types | serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range | 62.5 U/mL-4000 U/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human CD8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human CD8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human CD8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 94; 1: Range %; 2: 86-98; 1: 1:2; 2: Average %; 3: 99; 1: Range %; 2: 88-104; 1: 1:4; 2: Average %; 3: 97; 1: Range %; 2: 89-100; 1: 1:8; 2: Average %; 3: 105; 1: Range %; 2: 94-109 |
| Recovery | headers: The recovery of human CD8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human CD8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human CD8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; ; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 100; 3: 88-104; 1: EDTA plasma (n=4); 2: 97; 3: 88-100; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: U/ml OD1 OD2 Average Corrected 4000 1.979 1.941 1.960 1.783 2000 1.471 1.455 1.463 1.286 1000 0.961 0.989 0.975 0.798 500 0.647 0.685 0.666 0.489 250 0.447 0.454 0.451 0.274 125 0.291 0.308 0.300 0.123 62.5 0.227 0.232 0.230 0.053 0 0.179 0.175 0.177; 2: U/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 9: 4000; 10: 1.979; 11: 1.941; 12: 1.960; 13: 1.783; 16: 2000; 17: 1.471; 18: 1.455; 19: 1.463; 20: 1.286; 23: 1000; 24: 0.961; 25: 0.989; 26: 0.975; 27: 0.798; 30: 500; 31: 0.647; 32: 0.685; 33: 0.666; 34: 0.489; 37: 250; 38: 0.447; 39: 0.454; 40: 0.451; 41: 0.274; 44: 125; 45: 0.291; 46: 0.308; 47: 0.300; 48: 0.123; 51: 62.5; 52: 0.227; 53: 0.232; 54: 0.230; 55: 0.053; 58: 0; 59: 0.179; 60: 0.175; 61: 0.177; 1: U/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 4000; 2: 1.979; 3: 1.941; 4: 1.960; 5: 1.783; 1: 2000; 2: 1.471; 3: 1.455; 4: 1.463; 5: 1.286; 1: 1000; 2: 0.961; 3: 0.989; 4: 0.975; 5: 0.798; 1: 500; 2: 0.647; 3: 0.685; 4: 0.666; 5: 0.489; 1: 250; 2: 0.447; 3: 0.454; 4: 0.451; 5: 0.274; 1: 125; 2: 0.291; 3: 0.308; 4: 0.300; 5: 0.123; 1: 62.5; 2: 0.227; 3: 0.232; 4: 0.230; 5: 0.053; 1: 0; 2: 0.179; 3: 0.175; 4: 0.177 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human CD8A ELISA Kit was designed for the quantitative measurement of Human CD8A protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 U/mL-4000 U/mL and the sensitivity is 15.6 U/mL. |
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