Human Coenzyme Q10 (CoQ10) ELISA Kit CSB-E14081h CUSABIO
$50.00
In stock
SKU
CSB-E14081h
Product Details
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 1.56 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Abbreviation | CoQ10 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 3.12 ng/mL-50 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Metabolism |
| Assay Principle | quantitative |
| Measurement | Competitive |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human CoQ10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human CoQ10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human CoQ10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:20; 2: Average %; 3: 96; 1: Range %; 2: 91-101; 1: 1:40; 2: Average %; 3: 99; 1: Range %; 2: 93-104; 1: 1:80; 2: Average %; 3: 92; 1: Range %; 2: 87-96; 1: 1:160; 2: Average %; 3: 90; 1: Range %; 2: 85-94 |
| Recovery | headers: The recovery of human CoQ10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human CoQ10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human CoQ10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 84; 3: 80-88; 1: EDTA plasma (n=4); 2: 93; 3: 88-97; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: ng/ml OD1 OD2 Average 50 0.112 0.103 0.108 25 0.208 0.224 0.216 12.5 0.377 0.397 0.387 6.25 0.783 0.718 0.751 3.12 1.121 1.086 1.104 0 1.951 1.829 1.890; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 8: 50; 9: 0.112; 10: 0.103; 11: 0.108; 14: 25; 15: 0.208; 16: 0.224; 17: 0.216; 20: 12.5; 21: 0.377; 22: 0.397; 23: 0.387; 26: 6.25; 27: 0.783; 28: 0.718; 29: 0.751; 32: 3.12; 33: 1.121; 34: 1.086; 35: 1.104; 38: 0; 39: 1.951; 40: 1.829; 41: 1.890; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 1: 50; 2: 0.112; 3: 0.103; 4: 0.108; 1: 25; 2: 0.208; 3: 0.224; 4: 0.216; 1: 12.5; 2: 0.377; 3: 0.397; 4: 0.387; 1: 6.25; 2: 0.783; 3: 0.718; 4: 0.751; 1: 3.12; 2: 1.121; 3: 1.086; 4: 1.104; 1: 0; 2: 1.951; 3: 1.829; 4: 1.890 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | Coenzyme Q10 is a lipophilic quinone that plays an important role in cellular energy production within the mitochondrial electron transport chain. As a key component of oxidative phosphorylation, CoQ10 supports ATP synthesis and serves as a potent antioxidant, protecting cellular membranes from oxidative damage. This ubiquinone derivative is naturally synthesized in human tissues. Concentrations decline with age and in various pathological conditions, making it an important biomarker for metabolic health and mitochondrial function research. The Human Coenzyme Q10 ELISA Kit uses a competitive quantitative assay principle to measure CoQ10 levels in serum, plasma, and tissue homogenates. The kit offers a detection range of 3.12 ng/mL to 50 ng/mL with a sensitivity of 1.56 ng/mL, requiring 50-100 μL sample volumes for analysis. Detection occurs at 450 nm wavelength. Assay completion takes 1-5 hours, providing researchers with a reliable tool for CoQ10 quantification in human samples. Application Examples Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section. This ELISA kit has been used in research focused on mitochondrial function and vitamin supplementation studies. The kit allows quantification of CoQ10 levels in cellular and serum samples to evaluate mitochondrial health and monitor nutritional status. • Mitochondrial function research: Measurement of CoQ10 concentrations in cell culture systems to evaluate mitochondrial health and cellular bioenergetics • Nutritional supplementation studies: Monitoring of serum CoQ10 levels in clinical trials examining the effects of multivitamin supplementation over extended treatment periods • Cellular metabolism: Analysis of CoQ10 content in homogenized cell samples following various experimental treatments to understand metabolic responses |
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