Human Collagen Type Ⅲ,Col Ⅲ ELISA KIT CSB-E04799h CUSABIO
$50.00
In stock
SKU
CSB-E04799h
Product Details
| Target Name | Collagen Type Ⅲ,Col Ⅲ |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 3.12 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Collagen Type 3,Col 3,Collagen Type III,Col III |
| Abbreviation | Col Ⅲ |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 12.5 ng/mL-800 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Signal Transduction |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 92; 1: Range %; 2: 86-96; 1: 1:2; 2: Average %; 3: 102; 1: Range %; 2: 97-105; 1: 1:4; 2: Average %; 3: 92; 1: Range %; 2: 85-98; 1: 1:8; 2: Average %; 3: 97; 1: Range %; 2: 91-102 |
| Recovery | headers: The recovery of human Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 86; 3: 80-94; 1: EDTA plasma (n=4); 2: 97; 3: 92-103; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: ng/ml OD1 OD2 Average Corrected 800 2.850 2.963 2.907 2.778 400 2.255 2.203 2.229 2.100 200 1.279 1.256 1.268 1.139 100 0.659 0.655 0.657 0.528 50 0.438 0.475 0.457 0.328 25 0.256 0.242 0.249 0.120 12.5 0.184 0.172 0.178 0.049 0 0.126 0.132 0.129; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 800; 9: 2.850; 10: 2.963; 11: 2.907; 12: 2.778; 14: 400; 15: 2.255; 16: 2.203; 17: 2.229; 18: 2.100; 20: 200; 21: 1.279; 22: 1.256; 23: 1.268; 24: 1.139; 26: 100; 27: 0.659; 28: 0.655; 29: 0.657; 30: 0.528; 32: 50; 33: 0.438; 34: 0.475; 35: 0.457; 36: 0.328; 38: 25; 39: 0.256; 40: 0.242; 41: 0.249; 42: 0.120; 44: 12.5; 45: 0.184; 46: 0.172; 47: 0.178; 48: 0.049; 50: 0; 51: 0.126; 52: 0.132; 53: 0.129; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 800; 2: 2.850; 3: 2.963; 4: 2.907; 5: 2.778; 1: 400; 2: 2.255; 3: 2.203; 4: 2.229; 5: 2.100; 1: 200; 2: 1.279; 3: 1.256; 4: 1.268; 5: 1.139; 1: 100; 2: 0.659; 3: 0.655; 4: 0.657; 5: 0.528; 1: 50; 2: 0.438; 3: 0.475; 4: 0.457; 5: 0.328; 1: 25; 2: 0.256; 3: 0.242; 4: 0.249; 5: 0.120; 1: 12.5; 2: 0.184; 3: 0.172; 4: 0.178; 5: 0.049; 1: 0; 2: 0.126; 3: 0.132; 4: 0.129 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | Collagen Type III is a major fibrillar collagen that maintains the structural integrity of various tissues, particularly in skin, blood vessels, and internal organs. This collagen subtype is essential for tissue elasticity and is commonly found in reticular fibers that provide scaffolding for cellular organization. Col III is frequently studied in research related to wound healing, fibrosis, cardiovascular disease, and connective tissue disorders, as its levels can indicate tissue remodeling processes and pathological conditions. The Human Collagen Type III ELISA Kit (CSB-E04799h) uses a quantitative sandwich measurement principle to detect Col III in human serum, plasma, and tissue homogenates. This assay offers a detection range of 12.5 ng/mL to 800 ng/mL with a sensitivity of 3.12 ng/mL. The protocol requires 50-100 μL sample volume and can be completed within 1-5 hours, with detection performed at 450 nm wavelength. Application Examples Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section. This ELISA kit has been used in research examining collagen metabolism and tissue remodeling processes. Studies have applied the kit to quantify human type III collagen levels in contracture tissue samples obtained during arthroscopic surgical procedures, showing its application in orthopedic and musculoskeletal research contexts. • Tissue analysis: Measurement of collagen type III concentrations in contracture tissue specimens collected during arthroscopic surgery • Musculoskeletal research: Examination of collagen metabolism in pathological tissue conditions affecting joint function • Fibrosis studies: Analysis of collagen type III as part of comprehensive extracellular matrix protein profiling in tissue remodeling research |
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