Human Creatine Kinase MB isoenzyme,CK-MB ELISA Kit CSB-E05140h CUSABIO
$50.00
In stock
SKU
CSB-E05140h
Product Details
| Target Name | Creatine Kinase MB isoenzyme,CK-MB |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.22 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Abbreviation | CK-MB |
| Sample Types | serum, plasma, urine, tissue homogenates |
| Detection Range | 0.9 ng/mL-60 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cardiovascular |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 86; 1: Range %; 2: 82-91; 1: 1:2; 2: Average %; 3: 94; 1: Range %; 2: 89-100; 1: 1:4; 2: Average %; 3: 98; 1: Range %; 2: 91-104; 1: 1:8; 2: Average %; 3: 106; 1: Range %; 2: 102-112 |
| Recovery | headers: The recovery of human CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 93; 3: 88-99; 1: EDTA plasma (n=4); 2: 98; 3: 91-104 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 60; 2: 3.334; 3: 3.234; 4: 3.284; 5: 3.168; 1: 30; 2: 2.152; 3: 2.067; 4: 2.110; 5: 1.994; 1: 15; 2: 1.214; 3: 1.168; 4: 1.191; 5: 1.075; 1: 7.5; 2: 0.743; 3: 0.729; 4: 0.736; 5: 0.620; 1: 3.75; 2: 0.443; 3: 0.451; 4: 0.447; 5: 0.331; 1: 1.8; 2: 0.289; 3: 0.273; 4: 0.281; 5: 0.165; 1: 0.9; 2: 0.198; 3: 0.187; 4: 0.192; 5: 0.077; 1: 0; 2: 0.113; 3: 0.118; 4: 0.116 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human CK-MB ELISA Kit was designed for the quantitative measurement of Human CK-MB protein in serum, plasma, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.9 ng/mL-60 ng/mL and the sensitivity is 0.22 ng/mL. |
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