Human Creatine kinase M-type(CKM) ELISA kit CSB-EL005459HU CUSABIO
$50.00
In stock
SKU
CSB-EL005459HU
Product Details
| Target Name | creatine kinase, muscle |
| Uniprot No. | P06732 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 1.56 μU/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | CKM ELISA Kit; CKMM ELISA Kit; Creatine kinase M ELISA Kit; Creatine kinase M chain ELISA Kit; Creatine kinase M type ELISA Kit; Creatine kinase M-type ELISA Kit; Creatine kinase muscle ELISA Kit; Creatine kinase, muscle type ELISA Kit; KCRM_HUMAN ELISA Kit; M-CK ELISA Kit; MCK ELISA Kit; Muscle creatine kinase ELISA Kit |
| Abbreviation | CKM |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 3.12 μU/mL-200 μU/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Metabolism |
| Assay Principle | quantitative |
| Measurement | Competitive |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human CKM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human CKM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human CKM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:100; 2: Average %; 3: 84; 1: Range %; 2: 80-88; 1: 1:200; 2: Average %; 3: 98; 1: Range %; 2: 94-102; 1: 1:400; 2: Average %; 3: 90; 1: Range %; 2: 86-94; 1: 1:800; 2: Average %; 3: 106; 1: Range %; 2: 101-113 |
| Recovery | headers: The recovery of human CKM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human CKM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human CKM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; ; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 95; 3: 90-100; 1: EDTA plasma (n=4); 2: 89; 3: 85-93; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: μU/ml OD1 OD2 Average 200 0.155 0.161 0.158 100 0.272 0.260 0.266 50 0.443 0.455 0.449 25 0.706 0.682 0.694 12.5 1.045 1.007 1.026 6.25 1.248 1.293 1.271 3.12 1.642 1.714 1.678 0 2.055 2.159 2.107; 2: μU/ml; 3: OD1; 4: OD2; 5: Average; 9: 200; 10: 0.155; 11: 0.161; 12: 0.158; 16: 100; 17: 0.272; 18: 0.260; 19: 0.266; 23: 50; 24: 0.443; 25: 0.455; 26: 0.449; 30: 25; 31: 0.706; 32: 0.682; 33: 0.694; 37: 12.5; 38: 1.045; 39: 1.007; 40: 1.026; 44: 6.25; 45: 1.248; 46: 1.293; 47: 1.271; 51: 3.12; 52: 1.642; 53: 1.714; 54: 1.678; 58: 0; 59: 2.055; 60: 2.159; 61: 2.107; 1: μU/ml; 2: OD1; 3: OD2; 4: Average; 1: 200; 2: 0.155; 3: 0.161; 4: 0.158; 1: 100; 2: 0.272; 3: 0.260; 4: 0.266; 1: 50; 2: 0.443; 3: 0.455; 4: 0.449; 1: 25; 2: 0.706; 3: 0.682; 4: 0.694; 1: 12.5; 2: 1.045; 3: 1.007; 4: 1.026; 1: 6.25; 2: 1.248; 3: 1.293; 4: 1.271; 1: 3.12; 2: 1.642; 3: 1.714; 4: 1.678; 1: 0; 2: 2.055; 3: 2.159; 4: 2.107 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human CKM ELISA Kit was designed for the quantitative measurement of Human CKM protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 3.12 μU/mL-200 μU/mL and the sensitivity is 1.56 μU/mL. |
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