Human Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3(CDKN2A) ELISA kit CSB-EL005089HU CUSABIO
$50.00
In stock
SKU
CSB-EL005089HU
Product Details
| Target Name | cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4) |
| Uniprot No. | P42771 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 4.69 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | CDKN2A ELISA Kit; CDKN2 ELISA Kit; MTS1 ELISA Kit; Cyclin-dependent kinase inhibitor 2A ELISA Kit; Cyclin-dependent kinase 4 inhibitor A ELISA Kit; CDK4I ELISA Kit; Multiple tumor suppressor 1 ELISA Kit; MTS-1 ELISA Kit; p16-INK4a ELISA Kit; p16-INK4 ELISA Kit; p16INK4A ELISA Kit |
| Abbreviation | CDKN2A |
| Sample Types | serum, plasma, tissue homogenates, cell lysates |
| Detection Range | 18.75 pg/mL-1200 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cell Biology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human CDKN2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human CDKN2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human CDKN2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 106; 1: Range %; 2: 101-112; 1: 1:2; 2: Average %; 3: 96; 1: Range %; 2: 92-99; 1: 1:4; 2: Average %; 3: 92; 1: Range %; 2: 86-96; 1: 1:8; 2: Average %; 3: 94; 1: Range %; 2: 90-99 |
| Recovery | headers: The recovery of human CDKN2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human CDKN2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human CDKN2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; ; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 103; 3: 99-107; 1: EDTA plasma (n=4); 2: 93; 3: 85-96; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: pg/ml OD1 OD2 Average Corrected 1200 2.355 2.443 2.399 2.306 600 1.806 1.784 1.795 1.702 300 1.333 1.230 1.282 1.189 150 0.841 0.906 0.874 0.781 75 0.462 0.460 0.461 0.368 37.5 0.274 0.290 0.282 0.189 18.75 0.163 0.160 0.162 0.069 0 0.095 0.090 0.093; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 9: 1200; 10: 2.355; 11: 2.443; 12: 2.399; 13: 2.306; 16: 600; 17: 1.806; 18: 1.784; 19: 1.795; 20: 1.702; 23: 300; 24: 1.333; 25: 1.230; 26: 1.282; 27: 1.189; 30: 150; 31: 0.841; 32: 0.906; 33: 0.874; 34: 0.781; 37: 75; 38: 0.462; 39: 0.460; 40: 0.461; 41: 0.368; 44: 37.5; 45: 0.274; 46: 0.290; 47: 0.282; 48: 0.189; 51: 18.75; 52: 0.163; 53: 0.160; 54: 0.162; 55: 0.069; 58: 0; 59: 0.095; 60: 0.090; 61: 0.093; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 1200; 2: 2.355; 3: 2.443; 4: 2.399; 5: 2.306; 1: 600; 2: 1.806; 3: 1.784; 4: 1.795; 5: 1.702; 1: 300; 2: 1.333; 3: 1.230; 4: 1.282; 5: 1.189; 1: 150; 2: 0.841; 3: 0.906; 4: 0.874; 5: 0.781; 1: 75; 2: 0.462; 3: 0.460; 4: 0.461; 5: 0.368; 1: 37.5; 2: 0.274; 3: 0.290; 4: 0.282; 5: 0.189; 1: 18.75; 2: 0.163; 3: 0.160; 4: 0.162; 5: 0.069; 1: 0; 2: 0.095; 3: 0.090; 4: 0.093 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human CDKN2A ELISA Kit was designed for the quantitative measurement of Human CDKN2A protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.69 pg/mL. |
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