Human Decorin/Bone proteoglycan II (DCN) ELISA Kit CSB-E16522h CUSABIO
$50.00
In stock
SKU
CSB-E16522h
Product Details
| Target Name | decorin |
| Uniprot No. | P07585 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.97 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Bone proteoglycan II ELISA Kit; CSCD ELISA Kit; DCN ELISA Kit; DCN protein ELISA Kit; Decorin ELISA Kit; Decorin proteoglycan ELISA Kit; Dermatan sulphate proteoglycans II ELISA Kit; DKFZp686J19238 ELISA Kit; DSPG 2 ELISA Kit; DSPG2 ELISA Kit; PG 40 ELISA Kit; PG II ELISA Kit; PG S2 ELISA Kit; PG-S2 ELISA Kit; PG40 ELISA Kit; PGII ELISA Kit; PGS 2 ELISA Kit; PGS2 ELISA Kit; PGS2_HUMAN ELISA Kit; Proteoglycan core protein ELISA Kit; SLRR1B ELISA Kit; Small leucine rich protein 1B ELISA Kit |
| Abbreviation | DCN |
| Sample Types | serum, plasma, tissue homogenates, cell lysates |
| Detection Range | 3.9 pg/mL-250 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Signal Transduction |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human DCN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human DCN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human DCN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:10; 2: Average %; 3: 95; 1: Range %; 2: 91-99; 1: 1:20; 2: Average %; 3: 103; 1: Range %; 2: 98-106; 1: 1:40; 2: Average %; 3: 110; 1: Range %; 2: 106-114; 1: 1:80; 2: Average %; 3: 92; 1: Range %; 2: 89-97 |
| Recovery | headers: The recovery of human DCN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human DCN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human DCN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; ; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 84; 3: 81-89; 1: EDTA plasma (n=4); 2: 97; 3: 93-100; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: pg/ml OD1 OD2 Average Corrected 250 1.944 1.968 1.956 1.815 125 1.284 1.232 1.258 1.117 62.5 0.750 0.771 0.761 0.620 31.2 0.512 0.502 0.507 0.366 15.6 0.374 0.368 0.371 0.230 7.8 0.258 0.262 0.260 0.119 3.9 0.194 0.199 0.197 0.056 0 0.140 0.142 0.141; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 9: 250; 10: 1.944; 11: 1.968; 12: 1.956; 13: 1.815; 16: 125; 17: 1.284; 18: 1.232; 19: 1.258; 20: 1.117; 23: 62.5; 24: 0.750; 25: 0.771; 26: 0.761; 27: 0.620; 30: 31.2; 31: 0.512; 32: 0.502; 33: 0.507; 34: 0.366; 37: 15.6; 38: 0.374; 39: 0.368; 40: 0.371; 41: 0.230; 44: 7.8; 45: 0.258; 46: 0.262; 47: 0.260; 48: 0.119; 51: 3.9; 52: 0.194; 53: 0.199; 54: 0.197; 55: 0.056; 58: 0; 59: 0.140; 60: 0.142; 61: 0.141; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 250; 2: 1.944; 3: 1.968; 4: 1.956; 5: 1.815; 1: 125; 2: 1.284; 3: 1.232; 4: 1.258; 5: 1.117; 1: 62.5; 2: 0.750; 3: 0.771; 4: 0.761; 5: 0.620; 1: 31.2; 2: 0.512; 3: 0.502; 4: 0.507; 5: 0.366; 1: 15.6; 2: 0.374; 3: 0.368; 4: 0.371; 5: 0.230; 1: 7.8; 2: 0.258; 3: 0.262; 4: 0.260; 5: 0.119; 1: 3.9; 2: 0.194; 3: 0.199; 4: 0.197; 5: 0.056; 1: 0; 2: 0.140; 3: 0.142; 4: 0.141 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human DCN ELISA Kit was designed for the quantitative measurement of Human DCN protein in serum, plasma, tissue homogenates, cell lysates . It is a Sandwich ELISA kit, its detection range is 3.9 pg/mL-250 pg/mL and the sensitivity is 0.97 pg/mL. |
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