Human E-Selectin ELISA kit CSB-E04540h CUSABIO
$50.00
In stock
SKU
CSB-E04540h
Product Details
| Target Name | selectin E |
| Uniprot No. | P16581 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.078 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | SELE; ELAM1; E-selectin; CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; ELAM-1; Leukocyte-endothelial cell adhesion molecule 2; LECAM2; CD antigen CD62E |
| Abbreviation | SELE |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 0.312 ng/mL-20 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; 1: Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean(ng/ml) 2.382 2.629 2.463 2.564 2.592 2.546 SD 0.041 0.043 0.036 0.049 0.052 0.047 CV(%) 5.76 5.61 4.93 6.52 6.86 6.28; 3: Intra-Assay Precision; 4: Inter-Assay Precision; 5: Sample; 6: 1; 7: 2; 8: 3; 9: 1; 10: 2; 11: 3; 12: n; 13: 20; 14: 20; 15: 20; 16: 20; 17: 20; 18: 20; 19: Mean(ng/ml); 20: 2.382; 21: 2.629; 22: 2.463; 23: 2.564; 24: 2.592; 25: 2.546; 26: SD; 27: 0.041; 28: 0.043; 29: 0.036; 30: 0.049; 31: 0.052; 32: 0.047; 33: CV(%); 34: 5.76; 35: 5.61; 36: 4.93; 37: 6.52; 38: 6.86; 39: 6.28; 2: Intra-Assay Precision; 3: Inter-Assay Precision; 1: Sample; 2: 1; 3: 2; 4: 3; 5: 1; 6: 2; 7: 3; 1: n; 2: 20; 3: 20; 4: 20; 5: 20; 6: 20; 7: 20; 1: Mean(ng/ml); 2: 2.382; 3: 2.629; 4: 2.463; 5: 2.564; 6: 2.592; 7: 2.546; 1: SD; 2: 0.041; 3: 0.043; 4: 0.036; 5: 0.049; 6: 0.052; 7: 0.047; 1: CV(%); 2: 5.76; 3: 5.61; 4: 4.93; 5: 6.52; 6: 6.86; 7: 6.28 |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human E-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human E-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human E-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 99; 1: Range %; 2: 95-110; 1: 1:2; 2: Average %; 3: 95; 1: Range %; 2: 91-103; 1: 1:4; 2: Average %; 3: 87; 1: Range %; 2: 82-92; 1: 1:8; 2: Average %; 3: 94; 1: Range %; 2: 89-98; |
| Recovery | headers: The recovery of human E-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human E-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human E-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 95; 3: 89-99; 1: Heparin plasma (n=4); 2: 98; 3: 90-101; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; ; ; ; ; ; ; ; ; ; ; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 20; 2: 2.370; 3: 2.274; 4: 2.322; 5: 2.154; 1: 10; 2: 1.859; 3: 1.779; 4: 1.819; 5: 1.651; 1: 5; 2: 1.229; 3: 1.209; 4: 1.219; 5: 1.051; 1: 2.5; 2: 0.740; 3: 0.734; 4: 0.737; 5: 0.569; 1: 1.25; 2: 0.437; 3: 0.431; 4: 0.434; 5: 0.266; 1: 0.625; 2: 0.347; 3: 0.339; 4: 0.343; 5: 0.175; 1: 0.312; 2: 0.207; 3: 0.204; 4: 0.206; 5: 0.038; 1: 0; 2: 0.168; 3: 0.167; 4: 0.168; |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | E-selectin (SELE) is a cell adhesion molecule primarily expressed on activated endothelial cells in response to inflammatory stimuli such as cytokines and bacterial endotoxins. This glycoprotein plays an important role in the initial steps of leukocyte recruitment during inflammation by mediating the rolling and tethering of neutrophils, monocytes, and certain T-cell subsets along the vascular endothelium. E-selectin expression is rapidly upregulated during acute inflammatory responses. It serves as an important biomarker for endothelial activation and vascular inflammation in various pathological conditions. The Human E-Selectin ELISA kit (CSB-E04540h) is a quantitative sandwich immunoassay designed for measuring selectin E levels in human serum, plasma, and tissue homogenates. The assay offers a detection range of 0.312 ng/mL to 20 ng/mL with a sensitivity of 0.078 ng/mL. The protocol requires 50-100 μL sample volume and can be completed within 1-5 hours, with optical density measurements taken at 450 nm wavelength. Application Examples Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section. This ELISA kit has been used in clinical research studies to measure E-selectin levels in human biological samples as part of biomarker profiling. The kit supports research into vascular inflammation and endothelial dysfunction across various disease contexts. Researchers have used this assay alongside other inflammatory and vascular markers to measure disease-related changes in circulating biomarker levels. • Vascular inflammation research: Measuring E-selectin as a marker of endothelial activation in serum samples from patients undergoing clinical evaluation • Multi-biomarker profiling: Integration with panels measuring various inflammatory mediators, adhesion molecules, and vascular markers to provide disease characterization • Clinical biomarker studies: Measurement of circulating E-selectin levels in plasma samples as part of broader research into inflammatory and vascular pathways • Pre-surgical screening: Evaluation of E-selectin concentrations in patient serum samples collected prior to surgical procedures as part of biomarker screening protocols |
MDSD
Reviews
Write Your Own Review