Human Elafin(PI3) ELISA kit CSB-EL017952HU CUSABIO
$50.00
In stock
SKU
CSB-EL017952HU
Product Details
| Target Name | peptidase inhibitor 3, skin-derived |
| Uniprot No. | P19957 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 1.95 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Cementoin ELISA Kit; ELAF_HUMAN ELISA Kit; Elafin ELISA Kit; Elafin/Skalp ELISA Kit; Elastase Specific Inhibitor ELISA Kit; Elastase-specific inhibitor ELISA Kit; ES 1 ELISA Kit; ESI ELISA Kit; Peptidase inhibitor 3 ELISA Kit; Peptidase inhibitor 3; skin derived ELISA Kit; PI 3 ELISA Kit; PI-3 ELISA Kit; PI3 ELISA Kit; Pre elafin ELISA Kit; Protease inhibitor 3 skin derived ELISA Kit; Protease inhibitor 3; skin derived (SKALP) ELISA Kit; Protease inhibitor WAP3 ELISA Kit; SKALP ELISA Kit; Skin derived Anti leukoproteinase ELISA Kit; Skin-derived antileukoproteinase ELISA Kit; Trappin 2 ELISA Kit; WAP four disulfide core domain 14 ELISA Kit; WAP four disulfide core domain protein 14 ELISA Kit; WAP four-disulfide core domain protein 14 ELISA Kit; WAP3 ELISA Kit; WFDC14 ELISA Kit |
| Abbreviation | PI3 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 7.8 pg/mL-500 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Signal Transduction |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; 1: Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean(pg/ml) 62.974 63.991 62.090 61.964 63.482 61.586 SD 0.042 0.035 0.037 0.052 0.047 0.050 CV(%) 5.364 4.425 4.768 6.710 5.972 6.477; 3: Intra-Assay Precision; 4: Inter-Assay Precision; 5: Sample; 6: 1; 7: 2; 8: 3; 9: 1; 10: 2; 11: 3; 12: n; 13: 20; 14: 20; 15: 20; 16: 20; 17: 20; 18: 20; 19: Mean(pg/ml); 20: 62.974; 21: 63.991; 22: 62.090; 23: 61.964; 24: 63.482; 25: 61.586; 26: SD; 27: 0.042; 28: 0.035; 29: 0.037; 30: 0.052; 31: 0.047; 32: 0.050; 33: CV(%); 34: 5.364; 35: 4.425; 36: 4.768; 37: 6.710; 38: 5.972; 39: 6.477; 2: Intra-Assay Precision; 3: Inter-Assay Precision; 1: Sample; 2: 1; 3: 2; 4: 3; 5: 1; 6: 2; 7: 3; 1: n; 2: 20; 3: 20; 4: 20; 5: 20; 6: 20; 7: 20; 1: Mean(pg/ml); 2: 62.974; 3: 63.991; 4: 62.090; 5: 61.964; 6: 63.482; 7: 61.586; 1: SD; 2: 0.042; 3: 0.035; 4: 0.037; 5: 0.052; 6: 0.047; 7: 0.050; 1: CV(%); 2: 5.364; 3: 4.425; 4: 4.768; 5: 6.710; 6: 5.972; 7: 6.477; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human Elafin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human Elafin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human Elafin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:20; 2: Average %; 3: 89; 1: Range %; 2: 85-93; 1: 1:40; 2: Average %; 3: 100; 1: Range %; 2: 95-105; 1: 1:80; 2: Average %; 3: 87; 1: Range %; 2: 83-91; 1: 1:160; 2: Average %; 3: 105; 1: Range %; 2: 101-109; |
| Recovery | headers: The recovery of human Elafin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human Elafin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human Elafin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range%; 1: Serum (n=5); 2: 86; 3: 83-89; 1: EDTA plasma (n=4); 2: 99; 3: 95-104; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 500; 2: 2.783; 3: 2.901; 4: 2.842; 5: 2.678; 1: 250; 2: 1.852; 3: 1.798; 4: 1.825; 5: 1.661; 1: 125; 2: 1.210; 3: 1.205; 4: 1.208; 5: 1.044; 1: 62.5; 2: 0.779; 3: 0.789; 4: 0.784; 5: 0.620; 1: 31.2; 2: 0.483; 3: 0.478; 4: 0.481; 5: 0.317; 1: 15.6; 2: 0.331; 3: 0.354; 4: 0.343; 5: 0.179; 1: 7.8; 2: 0.279; 3: 0.268; 4: 0.274; 5: 0.110; 1: 0; 2: 0.165; 3: 0.163; 4: 0.164; |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
MDSD