Human Endostatin,ES ELISA Kit CSB-E07973h CUSABIO
$50.00
In stock
SKU
CSB-E07973h
Product Details
| Target Name | Endostatin,ES |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 3.9 pg/ml |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | FLJ27325, FLJ34914, KNO, KNO1, MGC74745, alpha 1 type XVIII collagen|antiangiogenic agent|endostatin|multi-functional protein MFP |
| Abbreviation | ES |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 15.6 pg/ml -1000 pg/ml |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cardiovascular |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human ES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human ES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human ES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:200; 2: Average %; 3: 87; 1: Range %; 2: 80-94; 1: 1:400; 2: Average %; 3: 93; 1: Range %; 2: 88-100; 1: 1:800; 2: Average %; 3: 92; 1: Range %; 2: 86-100; 1: 1:1600; 2: Average %; 3: 90; 1: Range %; 2: 86-98 |
| Recovery | headers: The recovery of human ES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human ES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human ES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 93; 3: 89-98; 1: EDTA plasma (n=4); 2: 94; 3: 88-100 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 1000; 2: 1.976; 3: 2.034; 4: 2.005; 5: 1.827; 1: 500; 2: 1.120; 3: 1.167; 4: 1.143; 5: 0.966; 1: 250; 2: 0.751; 3: 0.728; 4: 0.740; 5: 0.562; 1: 125; 2: 0.494; 3: 0.519; 4: 0.506; 5: 0.329; 1: 62.5; 2: 0.337; 3: 0.321; 4: 0.329; 5: 0.151; 1: 31.2; 2: 0.242; 3: 0.253; 4: 0.247; 5: 0.070; 1: 15.6; 2: 0.221; 3: 0.214; 4: 0.218; 5: 0.040; 1: 0; 2: 0.180; 3: 0.175; 4: 0.178 |
| Shelf Life | 6 months |
| Description | The product CSB-E07973h is a sandwich ELISA kit developed to measure levels of human endostatin (ES) in serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of endostatin bound in the initial step. Endostatin, a 20-kDa C-terminal cleavage product of type XVIII collagen, is an endogenous inhibitor known for its ability to inhibit the proliferation and migration of endothelial cells, angiogenesis, and tumor growth. Studies have proved that circulating endostatin increases significantly in patients with kidney and heart failure and may also contribute to disease progression. Endostatin inhibits angiogenesis by directly binding to both VEGFR1 and VEGFR2 and blocking VEGF interaction with Flt-1 and Flk-1 to prevent VEGF-induced tyrosine phosphorylation of VEGFR1 and VEGFR2 and all downstream signaling events. |
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