Human Eotaxin 2/CCL24 ELISA Kit CSB-E10360h CUSABIO
$50.00
In stock
SKU
CSB-E10360h
Product Details
| Target Name | chemokine (C-C motif) ligand 24 |
| Uniprot No. | O00175 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 67.660 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | C C motif chemokine 24 ELISA Kit; C-C motif chemokine 24 ELISA Kit; CCL24 ELISA Kit; CCL24_HUMAN ELISA Kit; Chemokine CC Motif Ligand 24 ELISA Kit; CK beta 6 ELISA Kit; CK-beta-6 ELISA Kit; Ckb6 ELISA Kit; Eosinophil chemotactic protein 2 ELISA Kit; Eotaxin-2 ELISA Kit; MPIF 2 ELISA Kit; MPIF-2 ELISA Kit; MPIF2 ELISA Kit; Myeloid progenitor inhibitory factor 2 ELISA Kit; SCYA24 ELISA Kit; Small inducible cytokine A24 ELISA Kit; Small inducible cytokine subfamily A (Cys-Cys) member 24 ELISA Kit; Small-inducible cytokine A24 ELISA Kit |
| Abbreviation | CCL24 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 78.125 pg/mL-5000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human eotaxin 2/CCL24 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human eotaxin 2/CCL24 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human eotaxin 2/CCL24 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 95; 1: Range %; 2: 90-100; 1: 1:2; 2: Average %; 3: 89; 1: Range %; 2: 85-94; 1: 1:4; 2: Average %; 3: 96; 1: Range %; 2: 91-101; 1: 1:8; 2: Average %; 3: 104; 1: Range %; 2: 100-108 |
| Recovery | headers: The recovery of human eotaxin 2/CCL24 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human eotaxin 2/CCL24 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human eotaxin 2/CCL24 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; ; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 99; 3: 95-103; 1: EDTA plasma (n=4); 2: 86; 3: 83-89; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: pg/ml OD1 OD2 Average Corrected 5000 2.152 2.204 2.178 2.065 2500 1.796 1.735 1.766 1.653 1250 1.182 1.205 1.194 1.081 625 0.765 0.798 0.782 0.669 312.5 0.453 0.468 0.461 0.348 156.25 0.328 0.331 0.330 0.217 78.125 0.251 0.240 0.246 0.133 0 0.115 0.110 0.113; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 9: 5000; 10: 2.152; 11: 2.204; 12: 2.178; 13: 2.065; 16: 2500; 17: 1.796; 18: 1.735; 19: 1.766; 20: 1.653; 23: 1250; 24: 1.182; 25: 1.205; 26: 1.194; 27: 1.081; 30: 625; 31: 0.765; 32: 0.798; 33: 0.782; 34: 0.669; 37: 312.5; 38: 0.453; 39: 0.468; 40: 0.461; 41: 0.348; 44: 156.25; 45: 0.328; 46: 0.331; 47: 0.330; 48: 0.217; 51: 78.125; 52: 0.251; 53: 0.240; 54: 0.246; 55: 0.133; 58: 0; 59: 0.115; 60: 0.110; 61: 0.113; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 5000; 2: 2.152; 3: 2.204; 4: 2.178; 5: 2.065; 1: 2500; 2: 1.796; 3: 1.735; 4: 1.766; 5: 1.653; 1: 1250; 2: 1.182; 3: 1.205; 4: 1.194; 5: 1.081; 1: 625; 2: 0.765; 3: 0.798; 4: 0.782; 5: 0.669; 1: 312.5; 2: 0.453; 3: 0.468; 4: 0.461; 5: 0.348; 1: 156.25; 2: 0.328; 3: 0.331; 4: 0.330; 5: 0.217; 1: 78.125; 2: 0.251; 3: 0.240; 4: 0.246; 5: 0.133; 1: 0; 2: 0.115; 3: 0.110; 4: 0.113 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This human CCL24 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human CCL24 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the CCL24 levels in the sample and is measured at 450 nm through a microplate reader. CCL24, also known as eotaxin2, mainly chemoattracts eosinophils. It can promote the separation of eosinophils from endothelial cells and then combine with its sole ligand to promote eosinophils to enter the tissue. CCL24 promotes cell trafficking and regulates inflammatory and fibrotic activities mainly through the CCR3. CCL24 induces chemotaxis and activation of CCR3-expressing cells, including immune cells and fibroblasts. A study found that CCL24 stimulated human lung fibroblast proliferation and collagen synthesis and was associated with the progression of idiopathic pulmonary fibrosis. Suppressing the expression of CCL24 can reduce airway inflammation. Macrophages can also selectively induce the production of CCL24. |
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