Human Epidermal growth factor,EGF ELISA Kit CSB-E08027h CUSABIO
$50.00
In stock
SKU
CSB-E08027h
Product Details
| Uniprot No. | P01133 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 1.95 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Beta urogastrone ELISA Kit; beta-urogastrone ELISA Kit; EGF ELISA Kit; EGF_HUMAN ELISA Kit; Epidermal growth factor ELISA Kit; HOMG4 ELISA Kit; OTTHUMP00000219721 ELISA Kit; OTTHUMP00000219722 ELISA Kit; Pro epidermal growth factor ELISA Kit; URG ELISA Kit; Urogastrone ELISA Kit |
| Abbreviation | EGF |
| Sample Types | serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range | 7.8 pg/ml-500 pg/ml. |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Others |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; 1: Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean(pg/ml) 63.799 65.356 60.135 62.315 64.315 64.315 SD 0.016 0.027 0.015 0.025 0.027 0.020 CV(%) 4.508 5.101 4.488 7.373 7.990 5.377; 3: Intra-Assay Precision; 4: Inter-Assay Precision; 5: Sample; 6: 1; 7: 2; 8: 3; 9: 1; 10: 2; 11: 3; 12: n; 13: 20; 14: 20; 15: 20; 16: 20; 17: 20; 18: 20; 19: Mean(pg/ml); 20: 63.799; 21: 65.356; 22: 60.135; 23: 62.315; 24: 64.315; 25: 64.315; 26: SD; 27: 0.016; 28: 0.027; 29: 0.015; 30: 0.025; 31: 0.027; 32: 0.020; 33: CV(%); 34: 4.508; 35: 5.101; 36: 4.488; 37: 7.373; 38: 7.990; 39: 5.377; 2: Intra-Assay Precision; 3: Inter-Assay Precision; 1: Sample; 2: 1; 3: 2; 4: 3; 5: 1; 6: 2; 7: 3; 1: n; 2: 20; 3: 20; 4: 20; 5: 20; 6: 20; 7: 20; 1: Mean(pg/ml); 2: 63.799; 3: 65.356; 4: 60.135; 5: 62.315; 6: 64.315; 7: 64.315; 1: SD; 2: 0.016; 3: 0.027; 4: 0.015; 5: 0.025; 6: 0.027; 7: 0.020; 1: CV(%); 2: 4.508; 3: 5.101; 4: 4.488; 5: 7.373; 6: 7.990; 7: 5.377 |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 102; 1: Range %; 2: 98-105; 1: 1:2; 2: Average %; 3: 98; 1: Range %; 2: 94-103; 1: 1:4; 2: Average %; 3: 96; 1: Range %; 2: 90-101; 1: 1:8; 2: Average %; 3: 93; 1: Range %; 2: 88-99; |
| Recovery | headers: The recovery of human EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 97; 3: 92-104; 1: Platelet-poor plasma (n=4); 2: 89; 3: 82-95; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 500; 2: 2.380; 3: 2.452; 4: 2.416; 5: 2.306; 1: 250; 2: 1.371; 3: 1.376; 4: 1.374; 5: 1.264; 1: 125; 2: 0.731; 3: 0.722; 4: 0.727; 5: 0.617; 1: 62.5; 2: 0.452; 3: 0.435; 4: 0.444; 5: 0.334; 1: 31.2; 2: 0.273; 3: 0.264; 4: 0.269; 5: 0.159; 1: 15.6; 2: 0.188; 3: 0.195; 4: 0.192; 5: 0.082; 1: 7.8; 2: 0.146; 3: 0.152; 4: 0.149; 5: 0.039; 1: 0; 2: 0.108; 3: 0.111; 4: 0.110; |
| Materials provided | A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human EGF antibody. This dismountable microplate can be divided into 12 x 8 strip plates. Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. One vial Biotin-labeled EGF antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody. One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic. One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody. One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution. One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration. One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances. One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation. An instruction manual |
| Materials not provided | A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. An incubator can provide stable incubation conditions up to 37°C±5°C. Centrifuge Vortex Squirt bottle, manifold dispenser, or automated microplate washer Absorbent paper for blotting the microtiter plate 50-300ul multi-channel micropipette Pipette tips Single-channel micropipette with different ranges 100ml and 500ml graduated cylinders Deionized or distilled water Timer Test tubes for dilution |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | The Human Epidermal growth factor (EGF) ELISA Kit is a sandwich immunoassay specifically designed and validated for the quantitative measurement of EGF in serum, plasma, cell culture supernates, or tissue homogenates. Human EGF is a single-chain polypeptide consisting of 53 amino acids. Physiologically in humans, EGF has different innate concentrations in various organs. EGF is implicated in the morphogenesis of teeth, brain, reproductive tracts, skin, and gastrointestinal tracts, in cardiovascular differentiation and function, epithelial regeneration, and corneal epithelia. EGF/EGFR signaling pathway regulates proliferation, migration, differentiation, and apoptosis in mammalian cells. Aberrations of the EGF/EGFR signaling pathway will lead to human cancer. This assay employs the Sandwich-ELISA technique, in which eNOS in the samples or standards is sandwiched between pre-coated an anti-human CgA and Biotin-labeled eNOS antibody. The Ag-Ab-Ag immune complex is labeled with HRP-avidin and then develops a color reaction after the addition of the TMB substrate. The intensity of the color is positively correlated to the amount of eNOS in the sample. The kit has high sensitivity, excellent specificity, precision low than 10%, and good lot-to-lot consistency. Get access to more details from the product instructions. |
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