Human Ferritin light chain(FTL) ELISA kit CSB-EL009049HU-IS CUSABIO
$50.00
In stock
SKU
CSB-EL009049HU-IS
Product Details
| Target Name | ferritin, light polypeptide |
| Uniprot No. | P02792 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 7.8pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days |
| Associated names | Ferritin L chain ELISA Kit; Ferritin L subunit ELISA Kit; Ferritin light chain ELISA Kit; Ferritin light polypeptide ELISA Kit; ferritin light polypeptide like 3 ELISA Kit; FRIL_HUMAN ELISA Kit; FTL ELISA Kit; LFTD ELISA Kit; NBIA 3 ELISA Kit; NBIA3 ELISA Kit |
| Abbreviation | FTL |
| Sample Types | serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range | 15.6pg/mL-1000pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Metabolism |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human FTL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human FTL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human FTL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:200; 2: Average %; 3: 85; 1: Range %; 2: 81-90; 1: 1:400; 2: Average %; 3: 95; 1: Range %; 2: 88-100; 1: 1:800; 2: Average %; 3: 97; 1: Range %; 2: 92-103; 1: 1:1600; 2: Average %; 3: 104; 1: Range %; 2: 99-109 |
| Recovery | headers: The recovery of human FTL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human FTL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human FTL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 98; 3: 92-105; 1: EDTA plasma (n=4); 2: 92; 3: 87-98 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 1000; 2: 2.307; 3: 2.234; 4: 2.270; 5: 2.106; 1: 500; 2: 1.314; 3: 1.397; 4: 1.355; 5: 1.191; 1: 250; 2: 0.901; 3: 0.839; 4: 0.870; 5: 0.706; 1: 125; 2: 0.499; 3: 0.532; 4: 0.515; 5: 0.351; 1: 62.5; 2: 0.285; 3: 0.307; 4: 0.296; 5: 0.132; 1: 31.2; 2: 0.218; 3: 0.223; 4: 0.220; 5: 0.056; 1: 15.6; 2: 0.194; 3: 0.184; 4: 0.189; 5: 0.025; 1: 0; 2: 0.167; 3: 0.161; 4: 0.164 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This ELISA kit provides quantitative measurement of Ferritin Light Chain (FTL) in human samples, supporting research into iron storage, metabolism, and ferritin assembly. Because FTL circulating levels span a broad dynamic range — from low picogram levels in iron-deficient states to elevated concentrations in inflammatory or iron-overload conditions — this kit is designed to capture biologically meaningful differences across diverse experimental models. Detection Range: The 15.6–1000 pg/mL range accommodates the low baseline FTL concentrations found in cell culture supernates and captures elevated levels seen in serum or plasma from iron-overload or inflammatory conditions, though samples from hemochromatosis or macrophage activation models may require dilution. Sensitivity: At 7.8 pg/mL, the kit detects FTL in conditioned media from hepatocytes or macrophages under basal culture conditions, where secreted ferritin levels can be minimal prior to iron or cytokine stimulation. Sample Compatibility: Validation across serum, plasma, cell culture supernates, and tissue homogenates enables researchers to correlate circulating FTL with intracellular ferritin pools in matched tissue or cell lysate samples — critical for dissecting iron sequestration dynamics at systemic and cellular levels. Assay Efficiency: The 1–5 hour protocol and 50–100 µL sample volume accommodate limited-volume primary hepatocyte or monocyte-derived macrophage cultures without pooling. This kit is well-suited for studies of hereditary hyperferritinemia-cataract syndrome, iron-deficiency anemia, and inflammation-driven ferritin dysregulation. |
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