Human Fetuin-B(FETUB) ELISA kit CSB-EL008598HU CUSABIO
$50.00
In stock
SKU
CSB-EL008598HU
Product Details
| Uniprot No. | Q9UGM5 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.137 ng/ml. |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | 16G2 ELISA Kit; 2310011O17Rik ELISA Kit; AI255764 ELISA Kit; D17980 ELISA Kit; Fet ELISA Kit; Fetub ELISA Kit; FETUB_HUMAN ELISA Kit; Fetuin beta ELISA Kit; Fetuin-B ELISA Kit; Fetuin-like protein ELISA Kit; Fetuin-like protein IRL685 ELISA Kit; Gugu ELISA Kit; IRL685 ELISA Kit; Pp63 ELISA Kit |
| Abbreviation | FETUB |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 0.137 ng/ml-100 ng/ml. |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cardiovascular |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; 1: Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean(ng/ml) 12.720 13.079 12.874 12.643 12.823 12.925 SD 0.023 0.027 0.025 0.036 0.033 0.031 CV(%) 3.622 4.160 3.900 5.696 5.164 4.821; 3: Intra-Assay Precision; 4: Inter-Assay Precision; 5: Sample; 6: 1; 7: 2; 8: 3; 9: 1; 10: 2; 11: 3; 12: n; 13: 20; 14: 20; 15: 20; 16: 20; 17: 20; 18: 20; 19: Mean(ng/ml); 20: 12.720; 21: 13.079; 22: 12.874; 23: 12.643; 24: 12.823; 25: 12.925; 26: SD; 27: 0.023; 28: 0.027; 29: 0.025; 30: 0.036; 31: 0.033; 32: 0.031; 33: CV(%); 34: 3.622; 35: 4.160; 36: 3.900; 37: 5.696; 38: 5.164; 39: 4.821; 2: Intra-Assay Precision; 3: Inter-Assay Precision; 1: Sample; 2: 1; 3: 2; 4: 3; 5: 1; 6: 2; 7: 3; 1: n; 2: 20; 3: 20; 4: 20; 5: 20; 6: 20; 7: 20; 1: Mean(ng/ml); 2: 12.720; 3: 13.079; 4: 12.874; 5: 12.643; 6: 12.823; 7: 12.925; 1: SD; 2: 0.023; 3: 0.027; 4: 0.025; 5: 0.036; 6: 0.033; 7: 0.031; 1: CV(%); 2: 3.622; 3: 4.160; 4: 3.900; 5: 5.696; 6: 5.164; 7: 4.821; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human Fetuin B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human Fetuin B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human Fetuin B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:10; 2: Average %; 3: 106; 1: Range %; 2: 102-108; 1: 1:20; 2: Average %; 3: 88; 1: Range %; 2: 84-92; 1: 1:40; 2: Average %; 3: 92; 1: Range %; 2: 85-96; 1: 1:80; 2: Average %; 3: 87; 1: Range %; 2: 82-83; |
| Recovery | headers: The recovery of human Fetuin B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human Fetuin B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human Fetuin B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range%; 1: Serum (n=5); 2: 104; 3: 98-108; 1: EDTA plasma (n=4); 2: 98; 3: 94-102; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 100; 2: 2.775; 3: 2.859; 4: 2.817; 5: 2.665; 1: 33.33; 2: 1.951; 3: 1.937; 4: 1.944; 5: 1.792; 1: 11.11; 2: 1.090; 3: 1.062; 4: 1.076; 5: 0.924; 1: 3.7; 2: 0.642; 3: 0.691; 4: 0.666; 5: 0.514; 1: 1.23; 2: 0.385; 3: 0.364; 4: 0.375; 5: 0.223; 1: 0.41; 2: 0.283; 3: 0.285; 4: 0.284; 5: 0.132; 1: 0.137; 2: 0.218; 3: 0.235; 4: 0.226; 5: 0.074; 1: 0; 2: 0.151; 3: 0.153; 4: 0.152; |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | The product CSB-EL008598HU is a sandwich ELISA kit developed to measure levels of human Fetuin-B (FETUB) in multiple samples, including serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of FETUB in samples. The color intensity is measured at 450 nm via a microplate reader. FETUB is a highly selective and potent inhibitor of metallopeptidases (MPs) of the astacin family. It is a liver-derived plasma protein with serum concentrations of about 0.01 g/l in humans. FETUB plays an essential role in female fertility. By blocking ovastacin, fetuin-B counteracts premature zona pellucida (ZP) hardening before fertilization and therefore maintains female fertility by keeping the ZP penetrable to sperm. Studies have found that FETUB is elevated in type 2 diabetes patients and FETUB knockdown improves glucose metabolism in obese mice. FETUB is also an inhibitor of basic calcium phosphate, blocking unwanted calcification. |
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