DIMT1 Polyclonal Antibody (E-AB-52600) elabscience
$399.00
In stock
SKU
E-AB-52600
200μL $ 399.00
Product Details
| Abbre | DIMT1 |
| Synonyms | 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase, 18S rRNA dimethylase, DIM1, DIM1 dimeth, DIM1 dimethyladenosine transferase 1 homolog, DIM1 dimethyladenosine transferase 1 homolog (S. cerevisiae), DIM1 dimethyladenosine transferase 1 like |
| Swissprot | Q9UNQ2 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB, IHC |
| Conjugation | Unconjugated |
| Concentration | 1.2 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Immunogen | Full length fusion protein |
| Calculated MW | 35 kDa |
| Observed MW | Refer to figures The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling |
| Cellular Localization | Nucleus>nucleolus. |
Product Description
Background: Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle.
Storage and Shipping
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Additional Information
| Verified Samples | Verified Samples in WB: 231 Verified Samples in IHC: Human prostate cancer, Human thyroid cancer |
| Dilution | WB 1:500-1:2000, IHC 1:30-1:150 |
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