GFP-Tag Monoclonal Antibody (E-AB-48011) elabscience
$130.00
In stock
SKU
E-AB-48011
100μL $ 130.00
Product Details
| Abbre | GFP-Tag |
| Synonyms | GFP, Green fluorescent protein |
| Swissprot | P42212 |
| Reactivity | All |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 5A2 |
| Isotype | IgG2a |
| Applications | WB, IF |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% Proclin300, 1% protective protein and 50% glycerol, pH7.4 |
| Purification Method | Protein A/G Purification |
| Immunogen | Recombinant GFP protein expressed by E. Coli |
| Calculated MW | 27 kDa |
| Observed MW | 27 kDa Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Product Description
Background: The green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. It is first isolated from the jellyfish Aequorea victoria and is frequently used as a reporter of expression. GFP is widely used in gene expression, tracing and protein subcellualr localization.
Storage and Shipping
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
Additional Information
| Verified Samples | Verified Samples in WB: 293T Ttrasfected with GFP-Tag, 293T |
| Dilution | WB 1:1000-2000, IF 1:500-2000 |
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