Glucocorticoid Receptor Polyclonal Antibody (E-AB-70157) elabscience
$410.00
In stock
SKU
E-AB-70157
200μL $ 410.00
Product Details
| Abbre | Glucocorticoid Receptor |
| Synonyms | GCCR, GCR, GCRST, GR, GRL, Glucocorticoid receptor, Grl1, Nuclear receptor subfamily 3 group C member 1, glucocorticoid nuclear receptor variant 1, group C, member 1 (glucocorticoid receptor), nr3c1, nuclear receptor subfamily 3 |
| Swissprot | P04150 P06536 P06537 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB, IHC |
| Conjugation | Unconjugated |
| Concentration | 1.3 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 1% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Immunogen | Recombinant proteincorresponding to Mouse Glucocorticoid receptor |
| Calculated MW | 91 kDa |
| Observed MW | 91 kDa Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling, Signal Transduction |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus. |
Product Description
Background: This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms.
Storage and Shipping
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Additional Information
| Verified Samples | Verified Samples in WB: Mouse heart, HepG2, MCF-7, Mouse Heart, Mouse kidney, Mouse brain, Rat heart, Rat kidney Verified Samples in IHC: Mouse liver, Rat spleen |
| Dilution | WB 1:500-1:1000, IHC 1:500-1:1000 |
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