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Luciferase Monoclonal Antibody (E-AB-22134) elabscience

Luciferase Monoclonal Antibody (E-AB-22134) elabscience

Name: Luciferase Monoclonal Antibody (E-AB-22134) elabscience
SKU: E-AB-22134
Price: 399 USD
Availability: InStock

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$399.00

In stock

SKU

E-AB-22134

Size	Price (USD)
200μL	$399.00
120μL	$240.00
60μL	$143.00
20μL	$73.00

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Product Details

Abbre	Luciferase
Synonyms	Firefly, Luciferase, Luciferin 4 monooxygenase, ec 1.13.12.7
Swissprot	P08659
Reactivity	Firefly
Host	Mouse
Clonality	Monoclonal
Clone	2C2
Isotype	IgG
Applications	WB, IF
Conjugation	Unconjugated
Concentration	1 mg/mL
Buffer	Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol.
Purification Method	Protein A purification
Immunogen	Recombinant Protein of Luciferase of Luciferase
Observed MW	60 kDa The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Research Areas	Tags & Cell Markers
Cellular Localization	Peroxisome, peroxisome.

Product Description

Background: Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Storage and Shipping

Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping	The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.

Additional Information