Recombinant Axin 2 Monoclonal Antibody (E-AB-81534) elabscience
$260.00
In stock
SKU
E-AB-81534
| Size | Price (USD) |
| 100μL | $260.00 |
| 50μL | $160.00 |
Product Details
| Abbre | Axin 2 |
| Synonyms | AXIL, AXIN2, ODCRCS, axin-2 |
| Swissprot | Q9Y2T1 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Rabbit Monoclonal |
| Clone | R06-3A8 |
| Isotype | IgG |
| Applications | WB |
| Conjugation | Unconjugated |
| Concentration | 300 μg/mL |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein. |
| Purification Method | Affinity Purified |
| Immunogen | A synthetic peptide of human Axin 2 |
| Calculated MW | 94 kDa |
| Observed MW | 94 kDa Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Research Areas | Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Neuroscience, Stem Cells |
| Cellular Localization | Cytoplasm. |
Product Description
Background: The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.
Storage and Shipping
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Additional Information
| Verified Samples | Verified Samples in WB: K562, C6, 3T3, Hela |
| Dilution | WB 1:500-1:1000 |
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