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Recombinant Cytochrome C Monoclonal Antibody (E-AB-81449) elabscience

Recombinant Cytochrome C Monoclonal Antibody (E-AB-81449) elabscience

Name: Recombinant Cytochrome C Monoclonal Antibody (E-AB-81449) elabscience
SKU: E-AB-81449
Price: 260 USD
Availability: InStock

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$260.00

In stock

SKU

E-AB-81449

Size	Price (USD)
100μL	$260.00
50μL	$160.00

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Product Details

Abbre	Cytochrome C
Synonyms	CYC, Cytochrome C, HCS, Somatic, THC4
Swissprot	P99999
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Rabbit Monoclonal
Clone	R09-6H3
Isotype	IgG
Applications	WB, IHC-P
Conjugation	Unconjugated
Concentration	300 μg/mL
Buffer	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein.
Purification Method	Affinity Purified
Immunogen	A synthetic peptide of human Cytochrome C
Calculated MW	12 kDa
Observed MW	14 kDa The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Research Areas	Cancer, Cell Biology, Cardiovascular, Metabolism, Signal Transduction
Cellular Localization	Mitochondrion matrix.

Product Description

Background: Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation,cytochrome c can be released from mitochondria into cytoplasm,resulting in caspase-3 activation and apoptosis. Measurement of cytochrome c release from the mitochondria is useful for detection of the onset of apoptosis in cells. In addition,cytochrome c can also leave cells and be detectable in extra-cellular medium of apoptotic cells and serum of cancer patients. The level of serum cytochrome c may serve as a prognostic maker during cancer therapy.

Storage and Shipping

Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping	The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.

Additional Information