Recombinant E Cadherin Monoclonal Antibody (E-AB-81424) elabscience
$260.00
In stock
SKU
E-AB-81424
| Size | Price (USD) |
| 100μL | $260.00 |
| 50μL | $160.00 |
Product Details
| Abbre | E Cadherin |
| Synonyms | Arc 1, CADH1, CAM 120/80, CD 324, CD324, CD324 antigen, CDHE, Cadherin 1, Cadherin1, E-Cad/CTF3, E-cadherin, ECAD, Epithelial cadherin, LCAM, Liver cell adhesion molecule, UVO, Uvomorulin, cadherin 1 type 1 E-cadherin, cdh1, epithelial calcium dependant adhesion protein |
| Swissprot | P12830 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Rabbit Monoclonal |
| Clone | R07-4F1 |
| Isotype | IgG |
| Applications | WB, IHC-P, IF |
| Conjugation | Unconjugated |
| Concentration | 300 μg/mL |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein. |
| Purification Method | Affinity Purified |
| Immunogen | A synthetic peptide of human E Cadherin |
| Calculated MW | 98 kDa |
| Observed MW | 130 kDa The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Research Areas | Cancer, Developmental Biology, Signal Transduction |
| Cellular Localization | Cell junction. Cell membrane. Endosome. Golgi apparatus>trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane. |
Product Description
Background: This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites.
Storage and Shipping
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Additional Information
| Verified Samples | Verified Samples in WB: PC3 Verified Samples in IF: Human Cholangiocarcinoma, MCF-7 |
| Dilution | WB 1:500-1:1000, IHC 1:100-1:200, IF 1:20-1:200 |
- A potent Bioorganic azapodophyllotoxin derivative Suppresses tumor Progression in Triple negative breast Cancer: An Insight into its Inhibitory effect on tubulin polymerization and Disruptive effect on microtubule assembly
Journal: BIOORGANIC CHEMISTRY | Year: 2024 | Impact Factor: 4.500
DOI: 10.1016/j.bioorg.2024.107839 | Reactivity: Human | Sample Type: MDA-MB-231 cell,MDA-MB-468 cell - Computational and in?vitro screening validates the repositioning potential of Coxibs as anti-fibrotic agents
Journal: JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS | Year: 2024 | Impact Factor: 4.400
DOI: 10.1080/07391102.2024.2318655 | Reactivity: Human | Sample Type: A549 cell - Expression of Markers Associated with Epithelial—Mesenchymal Transition and Extracellular Matrix Degradation in Human Uveal Melanoma
Journal: BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | Year: 2024 | Impact Factor: 0.900
DOI: 10.1007/s10517-024-06266-x | Reactivity: Human | Sample Type: choroid tissue without tumor - Hypermethylation of the GRHL2 promoter region is associated with ovarian endometriosis
Journal: REPRODUCTION | Year: 2022 | Impact Factor: 3.923
DOI: 10.1530/REP-21-0383 | Reactivity: E-AB-1196, Human | Sample Type: endometrial epithelial cells (EECs)
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