Recombinant Vimentin Monoclonal Antibody (E-AB-81438) elabscience
$260.00
In stock
SKU
E-AB-81438
| Size | Price (USD) |
| 100μL | $260.00 |
| 50μL | $160.00 |
Product Details
| Abbre | Vimentin |
| Synonyms | CTRCT30, Epididymis luminal protein 113, FLJ36605, HEL113, VIM, VIME, Vimentin |
| Swissprot | P08670 |
| Reactivity | Human, Mouse, Rat, Hamster |
| Host | Rabbit |
| Clonality | Rabbit Monoclonal |
| Clone | R02-8C4 |
| Isotype | IgG |
| Applications | WB, IHC-P, IF |
| Conjugation | Unconjugated |
| Concentration | 300 μg/mL |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein. |
| Purification Method | Affinity Purified |
| Immunogen | A synthetic peptide of human Vimentin |
| Calculated MW | 54 kDa |
| Observed MW | 54 kDa Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Research Areas | Cancer, Developmental Biology, Neuroscience, Signal Transduction, Stem Cells |
| Cellular Localization | Cytoplasm |
Product Description
Background: Vimentin,also named as VIM,belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells,especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092),suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein,55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.
Storage and Shipping
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Additional Information
| Verified Samples | Verified Samples in WB: C6, CHO-K1, Hela Verified Samples in IF: Human tonsil, Hela |
| Dilution | WB 1:500-1:1000, IHC 1:50-1:100, IF 1:50-1:100 |
- Simultaneous regulation of AGE/RAGE signaling and MMP-9 expression by an immunomodulating hydrogel accelerates healing in diabetic wounds
Journal: Biomaterials Advances | Year: 2024 | Impact Factor: 5.500
DOI: 10.1016/j.bioadv.2024.213937 | Reactivity: Rat | Sample Type: wound bed tissue - Computational and in?vitro screening validates the repositioning potential of Coxibs as anti-fibrotic agents
Journal: JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS | Year: 2024 | Impact Factor: 4.400
DOI: 10.1080/07391102.2024.2318655 | Reactivity: Human | Sample Type: A549 cell - Constructing Tumor Organoid-like Tissue for Reliable Drug Screening Using Liver-Decellularized Extracellular Matrix Scaffolds
Journal: ACS Omega | Year: 2024 | Impact Factor: 4.100
DOI: 10.1021/acsomega.3c09265 | Reactivity: Human | Sample Type: HepG2 cell - Expression of Markers Associated with Epithelial—Mesenchymal Transition and Extracellular Matrix Degradation in Human Uveal Melanoma
Journal: BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | Year: 2024 | Impact Factor: 0.900
DOI: 10.1007/s10517-024-06266-x | Reactivity: Human | Sample Type: choroid tissue without tumor - Hypermethylation of the GRHL2 promoter region is associated with ovarian endometriosis
Journal: REPRODUCTION | Year: 2022 | Impact Factor: 3.923
DOI: 10.1530/REP-21-0383 | Reactivity: E-AB-1196, Human | Sample Type: endometrial epithelial cells (EECs)
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