Detection Principle: Cu 2+ can be reduced to Cu + by protein in alkaline condition. Cu + can combine with BCA reagent and form purple complex, which has a maximum absorption peak at 562 nm. The absorbance value is proportional to the protein concentration. Therefore, the protein concentration can be calculated according to the OD value.

Assay / Application Information

Detection Method	Colorimetric method
Detection Range	0.0165-1 mg/mL
Sensitivity	0.0165 mg/mL
Sample Type	Serum,Plasma,Cell culture supernatant,Tissue,Cell
Sample Volume	20 μL
Precision	inter-assay CV: 4.5% \| intra-assay CV: 2.2%
Assay Time	40 min
Valid Period	12 months
Detection Instrument	Microplate reader (540-590 nm)
Other Reagents Required	Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)

Storage and Shipping

Storage

This product can be stored at room temperature for 12 months with shading light.

Sample Information

10% Mouse brain tissue homogenization	8-12
10% Mouse kidney tissue homogenization	8-12
Human serum	100-200
10% Rat liver tissue homogenization	15-20
10% Mouse heart tissue homogenization	8-12
Rat serum	100-200

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You're reviewing:BCA Protein Colorimetric Assay Kit (E-BC-K318-M) elabscience

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