Bradford Protein Colorimetric Assay Kit (E-BC-K168-M) elabscience
$200.00
In stock
SKU
E-BC-K168-M
500Assays $ 200.00
Product Details
| Synonyms | TP |
| Research Area | Amino Acids And Proteins , Liver And Renal Function |
Product Description
Detection Principle: Coomassie brilliant blue G-250 is red under the free state, and it has the maximum absorbance at 465 nm. When the Coomassie brilliant blue G-250 combined to protein, the compound will have the maximum at 595 nm. The absorbance value is directly proportional to the protein content, so the concentration of total protein can be calculated directly by measuring the OD value at 595 nm.
Assay / Application Information
| Detection Method | Colorimetric method |
| Detection Range | 0.046-0.6 mg/mL |
| Sensitivity | 0.046 mg/mL |
| Sample Type | Serum,Plasma,Animal tissue,Plant tissue,Cell |
| Sample Volume | 10 μL |
| Precision | inter-assay CV: 8.2% | intra-assay CV: 3.2% |
| Assay Time | 20 min |
| Valid Period | 12 months |
| Detection Instrument | Microplate reader (550-630 nm) |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
Storage and Shipping
| Storage | This product can be stored at 2-8°C/room temperature for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
Sample Information
| Human serum | 90-110 |
| Human plasma | 90-110 |
| Rabbit serum | 90-110 |
| Rat plasma | 90-110 |
| Chicken serum | 90-110 |
| 10% Mouse kidney tissue homogenate | 15-20 |
| 10% Mouse lung tissue homogenate | 15-20 |
| 10% Rat spleen tissue homogenate | 15-20 |
| 10% Rat heart tissue homogenate | 15-20 |
| 10% Rat liver tissue homogenate | 20-25 |
| 293T | 1 |
| HL-60 | 1 |
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