ARG1 Polyclonal Antibody (E-AB-70308) elabscience
$410.00
In stock
SKU
E-AB-70308
| Size | Price (USD) |
| 200μL | $410.00 |
| 120μL | $260.00 |
| 60μL | $160.00 |
Product Details
| Abbre | ARG1 |
| Synonyms | A I, ARG 1, ARGI1, Al, Arginase, Arginase 1, Arginase liver, Arginase type I, Arginase-1, Arginase1, Liver type arginase, Liver-type arginase, Type I arginase, arg1, liver |
| Swissprot | P05089 P07824 Q61176 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB |
| Conjugation | Unconjugated |
| Concentration | 40 μg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 1% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Immunogen | Recombinant protein corresponding to Mouse Arginase-1 |
| Calculated MW | 35 kDa |
| Observed MW | 40 kDa The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Research Areas | Cancer, Metabolism, Signal Transduction |
| Cellular Localization | Cytoplasm. |
Product Description
Background: Liver arginase(Arginase-1) belongs to the arginase family. Hepatic arginase 1 is closely associated with alternative macrophage activation(PMID:12098359) and Arg1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins(PMID: 20071539).It can exsit as a homotrimer(PMID:16141327) and it has 3 isoforms produced by alternative splicing.Defects in ARG1 are the cause of argininemia (ARGIN).
Storage and Shipping
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Additional Information
| Verified Samples | Verified Samples in WB: Human liver, Mouse liver, Rat liver |
| Dilution | WB 1:1000-1:2000 |
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