CFL1 Polyclonal Antibody (E-AB-40164) elabscience
$260.00
In stock
SKU
E-AB-40164
100μL $ 260.00
Product Details
| Abbre | Cofilin-1 |
| Synonyms | 18 kDa phosphoprotein, CFL, CFL 1, CFL1, COF1, Cofilin, Cofilin 1, Cofilin 1 non muscle, Cofilin non muscle isoform, Cofilin-1, HEL-S-15, epididymis secretory protein Li 15, non-muscle isoform, p18 |
| Swissprot | P23528 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% Proclin300, 1% protective protein and 50% glycerol, pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Immunogen | Recombinant human Homeobox protein Cofilin-1 protein |
| Calculated MW | 17 kDa |
| Observed MW | 17 kDa Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Research Areas | Cancer, Cardiovascular, Neuroscience, Signal Transduction |
| Cellular Localization | Nucleus matrix. Cytoplasm>cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide. |
Product Description
Background: The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.
Storage and Shipping
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Additional Information
| Verified Samples | Verified Samples in WB: A431, Jurkat |
| Dilution | WB 1:500-1:1000 |
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