The conventional cell culture is performed using 2D planes, and the growth model of 2D cell cultures is very different from the 3D environment in vivo. This causes significant differences in cellular morphology, cell differentiation, cell-matrix interaction and intercellular interaction when compared to the behavior under physiological conditions in vivo. A 3D cell culture provides an ideal simulated environment for an in vivo pattern of cell growth. The 3D cell culture scaffold produced by JET BIOFIL (patent number: ZL201620728244.6, ZL201620728243.1 and 201510783345.3) is an ideal tool for studying 3D cell cultures, the mechanism of interaction between cells, cellular immunotherapy and stem cell therapy, drug screening, as well as drug production. Furthermore, it improves the cell culture area and increases the yield significantly. This 3D cell culture scaffold can be used with 6, 12, 24 well culture plates and culture dishes of different sizes such as 35 mm, 60 mm and 70 mm.
Features
Average wire diameter: 500µm; average spacing of wire column: 260 µm, with high regularity. The product consists of a 3D porous structure with good connectivity, facilitating the transmission of different nutritional ingredients in the course of the 3D cell culture, and ensuring the consistency of metabolic activity and accuracy of culture results
In comparison to the 2D cell culture, the 3D cell culture allows for easier cell function expression since it simulates the 3D structure of human and animal cells to a maximum level and provides an ideal interactive environment between cells
The 3D cell culture scaffold has a much larger culture surface area than conventional 2D cell culture products, thus saving on both space and material, and significantly improving cell culture efficiency and yield
Cells adhere strongly to the surface because of the advanced hydrophilic treatment
No adsorption of cytokines or growth factors; cell and cell secretions can be directly isolated from the 3D scaffold when harvesting
The 3D scaffold has a four layered three-dimensional structure with a highly hydrophilic surface for adherent culture. The 3D scaffold is built into the culture plate well or culture dish.
Structural diagram of 3D cell culture scaffold: fibers between each layer alternate at 90°, and fibers parallel and not opposite between each 2 layers.