10×Capping Reaction Buffer, GMP Grade(Cat. No.:GMP-EB62) novoprotein
$17.00
In stock
SKU
GMP-EB62
1ml: $17
5ml: Inquiry
10ml: Inquiry
50ml: Inquiry
100ml: Inquiry
Description
10×Capping Reaction Buffer, GMP Grade is the companion reaction buffer for the Vaccinia Capping Enzyme, GMP Grade (Cat. No.: GMP-M062) and mRNA Cap 2´-O-Methyltransferase, GMP Grade (Cat. No.: GMP-M072). The formula of 1×Capping Reaction Buffer is 50mM Tris-HCl (pH 8.0 at 25℃), 5mM KCl, 1mM DTT, 1mM MgCl<sub>2</sub>.
Quality Control
| Element | Standard |
| Appearance | Clear and transparent solution |
| Visible Particles | Meet the specification |
| pH | 7.5-8.5 |
| Endonuclease Residues | The degradation of substrate was ≤10% |
| Exonuclease Residues | The degradation of substrate was ≤10% |
| RNase Residues | The degradation of substrate was ≤10% |
| Non-Specific DNase Residues | The degradation of substrate was ≤10% |
| Bacterial Endotoxins | ＜1EU/ml |
| Mycoplasma | Negative |
| Heavy Metals | ≤10ppm |
| Microbial Limit | Total aerobic microbial count≤1cfu/10ml, total yeasts and molds count≤1cfu/10ml |
Product Details
| Item | Details |
| Storage | At -20±5℃. |
Components
| SKU | Components | Volume |
| GMP-EB62-01A | 10×Capping Reaction Buffer, GMP Grade | 1ml |
| GMP-EB62-M005 | 10×Capping Reaction Buffer, GMP Grade | 5ml |
| GMP-EB62-M010 | 10×Capping Reaction Buffer, GMP Grade | 10ml |
| GMP-EB62-M050 | 10×Capping Reaction Buffer, GMP Grade | 50ml |
Notes
Please mix upside down gently (avoid foaming) and slight centrifugation before use. For the preparation and dispensing of the reaction solution, please use a new (RNase-free) pipette tip, microtube, etc., to ensure that all reagents are free of RNase pollution.
To ensure the efficient reaction, high-quality DNA template should be used and operated on ice.
For your safety and health, please wear a lab coat and disposable gloves.
Related Products
| Cat. No. | Product Name | Cat. No. | Product Name |
| GMP-M062 | Vaccinia Capping Enzyme, GMP Grade | GMP-E125 | RNase Inhibitor, GMP Grade |
| GMP-M072 | mRNA Cap 2´-O-Methyltransferase, GMP Grade | GMP-E127 | DNase I, GMP Grade |
| GMP-M012 | E. coli Poly(A) Polymerase, GMP Grade | GMP-M036 | Pyrophosphatase, Inorganic (yeast), GMP Grade |
| GMP-E121 | T7 RNA Polymerase, GMP Grade | GMP-E131 | T7 RNA Transcription Enzyme Mix, GMP Grade |
| GMP-S023-026 | NTPs, GMP Grade |
title: On-column capping of poly dT media-tethered mRNA accomplishes high capping efficiency, enhanced mRNA recovery, and improved stability against RNase
authors: Shiyi Che, Xue Feng, Zhengjun Li, Zhiguo Su, Guanghui Ma, Zhikao Li, Aibing Yu, Minsu Liu, Songping Zhang
date: 2023-09-25
journal: BIOTECHNOLOGY AND BIOENGINEERING
MDSD
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