BsaI, GMP Grade(Cat. No.:GMP-RE026) novoprotein
$50.00
In stock
SKU
GMP-RE026
1000U: Inquiry
10KU: Inquiry
100KU: Inquiry
500KU: Inquiry
Description
Restriction endonucleases are a class of endonuclease that recognize specific deoxynucleotide sequences and cleave the phosphodiester bond between two deoxyribonucleotides at specific sites in each chain. BsaI derived from Bacillus Thermophilus, is a commonly used restriction endonuclease. BsaI recognizes the following sequence:
5’…GGTCTC(N)1↓…3’;
3’…CCAGAG(N)5↑…5’.
Our manufacturing processes are strictly controlled to ensure the end products free from host protein or nucleic acid contaminations and other impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing.
This product has completed the DMF record of FDA.
Quality Control
| Element | Standard |
| Appearance | Clear and transparent solution |
| Visible Particles | Meet the specification |
| pH | 7.0-8.0 |
| Activity | 10KU/ml-10.5KU/ml |
| Endonuclease Residues | The degradation of substrate was ≤10% |
| Exonuclease Residues | The degradation of substrate was ≤10% |
| RNase Residues | The degradation of substrate was ≤10% |
| Bacterial Endotoxins | <1EU/ml |
| Heavy Metal Residues | ≤10ppm |
| Microbial Limit | Total aerobic microbial count ≤1cfu/10ml, total yeasts and molds count≤1cfu/10ml |
Product Details
| Item | Details |
| Storage | At -70±10℃ |
Components
| SKU | Components | Volume |
| GMP-RE026-01A | BsaI, GMP Grade (10U/μl) | 100μl |
| GMP-RE026-M001 | BsaI, GMP Grade (10U/μl) | 1ml |
| GMP-RE026-M010 | BsaI, GMP Grade (10U/μl) | 10ml |
| GMP-RE026-M050 | BsaI, GMP Grade (10U/μl) | 50ml |
Notes
- BsaI is sensitive to some methylation modifications. When using it, pay attention to the substrates without BsaI-sensitive methylation modifications. Effects of methylation modification:
- Affected by CpG methylation and Dcm methylation, the sequences were completely overlapped, and shear blocked.
- Due to EcoBI methylation, the sequence overlaps completely, and the shear may be blocked.
- It is not affected by Dam methylation, the sequence is not overlapped, and the shear is not blocked.
- If the digested product is used as a template for in vitro transcription, it is recommended to use it after purification.
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title: AntigenBoost: enhanced mRNA-based antigen expression through rational amino acid substitution
authors: Gao Yumiao, Zhu Siran, Li Huichun, Hao Xueting, Chen Wen, Pan Deng, Qian Zhikang
date: 2024/10/14
journal: BRIEFINGS IN BIOINFORMATICS
MDSD
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