BenzoNuclease® (Tag-free), GMP Grade(Cat. No.:GMP-1709) novoprotein
$630.00
In stock
SKU
GMP-1709
100KU: $630
500KU: Inquiry
5000KU: Inquiry
Description
BenzoNuclease® is a recombinant endonuclease derived from Serratia marcescensa. The protein is expressed in Escherichia coli (E. coli). BenzoNuclease® has been shown to exert a broad spectrum of substrate specificity to degrade both DNA and RNA—whether single-stranded, double-stranded, linear, circular or supercoiled. No base preference is observed. As with all endonucleases, BenzoNuclease® hydrolyzes internal phosphodiester bonds present between the nucleotides. Upon complete digestion, all free nucleic acids present in solution are reduced to 5’-monophosphate-terminated oligonucleotides, which are three to five bases in length.
In scientific research, BenzoNuclease® can be used as the preferred enzyme preparation for supernatant of cultured cells and viscosity removal of cell lysate, which can remove nucleic acid interference and improve the subsequent protein purification or functional research. At the same time in the vaccine industry, protein and polysaccharide pharmaceutical industry, used to remove the host residual nucleic acid, greatly reduce the vaccine and protein products of nucleic acid pollution, reduce the processing steps, improve the yield of products.
This product is expressed by large-scale fermentation of E. coli, and is produced with raw materials of medicinal specifications. The host protein residue, nucleic acid residue and common pathogens are strictly controlled, and the production and quality management procedures of the product comply with GMP regulations to ensure the traceability of the production process and all raw materials.
Usage
Used to remove exogenous nucleic acid from vaccine products, reduce the risk of residual toxicity of nucleic acid and improve product safety.
Used to reduce the viscosity of feed liquid caused by nucleic acid, shorten the processing time and increase the protein yield.
Used to remove nucleic acid winding on the surface of particles (viruses, inclusion bodies, etc.), which is conducive to the release and purification of particles.
Used to prepare samples for ELISA, column chromatography, 2D electrophoresis and Western blot analysis. The resolution and recovery can be improved after BenzoNuclease® treatment.
Used to prevent cell clumping.
Quality Control
| Element | Standard |
| Appearance | clear, transparent solution |
| Visible Particles | meet the specification |
| pH | 7.5-8.5 |
| Activity | 250-400 U/μl |
| Purity | ≥99% |
| Spec. Activity | ≥1.1×10<sup>6</sup> U/mg |
| Protease Activity | no protease activity detectable |
| Bacterial Endotoxins | < 0.01 EU/KU |
| Host-cell Protein Residues | ≤0.005% |
| Sterility | meet the specification |
| Mycoplasma | negative |
| Heavy Metal Residue | ≤ 10 ppm |
Product Details
| Item | Details |
| Storage | -20±5℃ |
Components
| SKU | Size | Item | Quantity |
| GMP-1709-100KU | 100KU | BenzoNuclease® (Tag-free), GMP Grade (250U/μl) | 400μl |
| GMP-1709-500KU | 500KU | BenzoNuclease® (Tag-free), GMP Grade (250U/μl) | 2ml |
| GMP-1709-5000KU | 5000KU | BenzoNuclease® (Tag-free), GMP Grade (250U/μl) | 20ml |
Product Details
| Item | Details |
| Activity Definition | In a 2.625 mL reaction system at 37℃ and pH 8.0, one unit of BenzoNuclease® is defined as the amount of enzyme that causes a change in absorbance at 260 nm of 1.0 absorption units within 30 minutes. |
Recommendations
Reference Dosage:
| Usage scenarios | Preparation of electrophoretic protein samples | Protein production | Production of vaccines and viruses | Cell medicine |
| Number of cells | 1×106 cells (10mg tissue) | 1g wet weight (10ml resuspension) | 1L fermentation supernatant | 1L cultures |
| Minimum dosage | 125 U | 250 U | 100 U | 100U |
| Recommended dosage | 500 U | 2500 U | 25000 U | 5000U |
Time: Normally the action time is 37℃ for 15~60 min, 25℃ for 30~120 min.
Reaction Condition:
| Condition | Optimal* | Effective** |
| Mg<sup>2+</sup> | 1-2mM | 1-10mM |
| pH | 8.0-9.2 | 6.0-10.0 |
| Temperature | 37℃ | 0-42℃ |
| DTT | 0-100mM | >100mM |
| BME | 0-100mM | >100mM |
| Na<sup>+</sup>, K<sup>+</sup>, etc. | 0-20mM | 0-150mM |
| PO<sub>4</sub><sup>3-</sup> | 0-10mM | 0-100mM |
*: Optimal is defined as the condition under which BenzoNuclease® retains >90% of its activity.
**: Effective is defined as the condition under which BenzoNuclease® retains >15% of its activity.
Notes
For sample repackaging, please use sterile pyrogen-free bottles with medical device certificates. Generally, Eppendorf tubes on the market are not medical devices.
The formulations of Novoprotein products have been optimized, and generally samples stored at 4°C for two weeks do not affect their biological activity, but long-term storage at 4°C is not recommended.
MDSD
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