Recombinant Cas9 Nuclease, GMP Grade(Cat. No.:GMP-1701) novoprotein
$50.00
In stock
SKU
GMP-1701
100μg: Inquiry
1mg: Inquiry
Description
CRISPR-Cas9 is an adaptive immune system developed by bacteria and archaea during the long-term evolution. The CRISPR-Cas9 system integrates fragments of invaded phage and plasmid DNA into CRISPR sequences and uses the corresponding CRISPR RNAs (crRNAs) to guide the cutting of homologous sequences by Cas9 protein and the system has immune memory ability. The artificially modified Cas9/sgRNA system can guide Cas9 protein to recognize and cut double-stranded DNA with sgRNA targets through sgRNA (single guide RNA), which can be used for gene knockout and precise DNA editing.
The recombinant S. pyogenes Cas9 protein expressed in E. coli which is cultured in animal-free medium and the protein is purified by multi-step column chromatography. It is produced with pharmaceutical raw materials and the host-cell protein residues and nucleic acid residues are strictly controlled. Product production and quality control conform to GMP standards, ensure the production process and all raw materials traceable.
FDA DMF
Usage
- Non-viral vector CRISPR gene knockout based on protein and sgRNA transfection.
- Non-viral vector CRISPR gene knock-in based on protein and sgRNA transfection.
Product Details
| Item | Details |
| Features | 1)Genetically engineered production without animal ingredients. 2)Preparation of raw materials and accessories for pharmaceutical specifications. |
Quality Control
| Element | Standard |
| Appearance | clear, transparent solution |
| Visible Particles | meet the specification |
| pH | 7.5-8.5 |
| Purity | ≥ 95% as analyzed by SDS-PAGE ≥ 95% as analyzed by SEC-HPLC |
| Molecular Weight | 163±16.3kDa (Reduced type) |
| Protein Content | 9.5~10.5mg/ml (Bradford assay) |
| Activity | In vitro cutting efficiency≥90% (300ng) |
| Bacterial Endotoxins Residues | < 0.01 EU/KU |
| Host-bacterial Protein Residues | ≤0.005% of total protein |
| Exogenous DNA Residues | ≤10ng per 1mg of protein |
| Endonuclease Residues | The degradation of substrate was ≤10% |
| Exonuclease Residues | The degradation of substrate was ≤10% |
| RNase Residues | The degradation of substrate was ≤10% |
| Mycoplasma | Negative (Mycoplasma kit) |
| Heavy Metal Residue | ≤ 10 ppm |
| Sterility | meet the specification |
Components
| Components | GMP-1701-U100 Size: 100μg | GMP-1701-M001 Size: 1mg |
| Recombinant Cas9 Nuclease, GMP Grade (10mg/ml) | 100μg | 1mg |
Product Details
| Item | Details |
| Storage | Store at -20±5℃, avoid repeated freezing and thawing; Valid for 2 years; |
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