T4 RNA Ligase 2(Cat. No.:M050) novoprotein
$50.00
In stock
SKU
M050
1000U: Inquiry
5000U: Inquiry
Description
T4 RNA Ligase 2 is an ATP-dependent double-strand RNA ligase (dsRNA ligase). It can be used for intramolecular cyclic ligation and intermolecular linear ligation of double-stranded RNA, and can also be used for intramolecular or intermolecular RNA ligation of double-stranded nucleic acids (RNA duplex, RNA/DNA hybrid strand, or DNA duplex) for the 3' hydroxyl group of the RNA strand and the 5' phosphate group of the DNA strand. T4 RNA Ligase 2 requires the presence of 5' phosphate and 3' hydroxyl groups for ligation, which can be a ligation reaction between the 5' phosphate group present in the RNA strand or DNA strand and the 3' hydroxyl group present in the RNA strand. Treatment at 65 °C for 15 min or boiling for 2 min can inactivate the enzyme.
Product Details
| Item | Details |
| Storage | Store at -20 °C ± 5 °C to avoid repeated freeze-thaw cycles. |
Components
| Component | M050-U100 | M050-U500 |
| T4 RNA Ligase 2 (10U/μl) | 100μl | 500μl |
| 10×T4 RNA Ligase 2 Reaction Buffer | 500μl | 1ml×2 |
| 50%PEG 8000 | 500μl | 1ml×2 |
Notes
For longer and more complex oligonucleotides, incubation at 25 °C for 2 h and then overnight at 16 °C may increase yield;
The 10 × T4 RNA Ligation 2 Reaction Buffer in the product already contains ATP and there is no need to add it again.
MDSD
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