Recombinant Human Beta-Actin (C-6His)(Cat. No.:CE15) novoprotein
$150.00
In stock
SKU
CE15
10μg: $150
50μg: $440
500μg: $1540
1mg: $2200
Description
Recombinant Human Beta-Actin is produced by our E.coli expression system and the target gene encoding Asp2-Phe375 is expressed with a 6His tag at the C-terminus.
Product Details
| Item | Details |
| Known As | Actin Cytoplasmic 1; Beta-Actin; ACTB |
| Mol Mass | 42.8 KDa |
| APMol Mass | 43 KDa, reducing conditions |
| Formulation Description | Supplied as a 0.2 μm filtered solution of 10mM Tris-HCl, 0.1% TritonX-100, 2mM DTT, 10% Glycerol, pH 8.0. |
| Endotoxin | < 1 EU/µg as determined by LAL test. |
| Storage | Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| Shipping | The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below. |
Background
Actins are ubiquitous globular and highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. ACTB is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.
title: Knockout of ACTB and ACTG1 with CRISPR/Cas9(D10A) Technique Shows that Non-Muscle β and γ Actin Are Not Equal in Relation to Human Melanoma Cells’ Motility and Focal Adhesion Formation
authors: Natalia Malek, Ewa Mrówczyńska, Aleksandra Michrowska, Ewa Mazurkiewicz, Iuliia Pavlyk, Antonina Joanna Mazur
date: 2020
journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
MDSD
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