| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.0-7.6 |
| Animal Origin Ingredient | Without |
Green Features
Sustainable packaging
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
Background
Ham's F-10 Nutrient Mixture is a culture medium designed by Ham for CHO cell serum-free culture in 1963. Ham's F-10 contains rich nutrients and is suitable for human diploid cells culture, cloning culture of cells under low serum conditions, normal culture of mammalian cells, chromosome analysis of white blood cells, and primary cell culture of rats, rabbits, chickens and other animals.
This product contains amino acids, vitamins, inorganic salts and other ingredients required for various types of cell culture, but does not contain proteins, lipids or any growth factors. So this product should be used with serum or serum-free additives.
Ingredient Statement
| With |
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| Without |
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Matters Need Attention
- This product is only used for scientific research or further research, not for diagnosis and treatment.
- This product is sterilized by 0.1 μm filtration.
- It is necessary to pay attention to the aseptic operation and avoid the contamination.
System Certification
GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's Ham's F-10 complies with ISO 13485, ISO 9001.
Q:1 If the basic culture medium is frozen at -20℃, will it affect its use?
If the cell culture medium is accidentally frozen, observe whether there is precipitation after the medium is naturally thawed. If no precipitation is produced, the medium can be used normally. If precipitation occurs, it is recommended to discard it.
Q:2 How long can I keep my culture media after supplementing with serum?
Generally speaking, media can be used for up to 3-6 months after supplementation with serum， at 2 to 8℃ low temperature environment preservation.
Q:3 My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?
We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.
Q:4 How light sensitive is media? Should I also be protecting it from LED light?
While we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.When placed in a light-proof 4°C refrigerator with the door closed, the culture medium will be kept away from light.
Q:5 I see a precipitate in my medium, and a change in the pH. What should I do?
This is most likely a sign of bacterial or fungal contamination. We would recommend discarding the media, and trying to decontaminate the culture.
Q:6 Where can I find the osmolality for Medium?
The osmolality is listed in the COA for the particular lot number of the medium.
Q:7 Do you provide customized culture media services?
Yes. We can customize culture media with reduced components or changes in the concentration of existing components.
Publications
- B cell deficiency limits exercise capacity by remodeling liver glutamate metabolism CELL; 2026; Impact Factor: 42.5; DOI: 10.1016/j.cell.2026.03.039
- Pristimerin disrupts blood-testis barrier (BTB) integrity via ferroptosis and apoptosis by the NUR77/ATF3/CHOP pathway. CHEMICO-BIOLOGICAL INTERACTIONS; 2026; Impact Factor: 5.2; DOI: 10.1016/j.cbi.2026.112255
- Formononetin Mitigates Age-Related Sarcopenia by Blocking Mitochondrial Ferroptosis via SIRT1/PGC-1α Signaling INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY; 2026; Impact Factor: 3.5; DOI: 10.1016/j.biocel.2026.107014
- RNF186 controls glucose metabolism in metabolic dysfunction-associated fatty liver disease Cell and Bioscience; 2025; Impact Factor: 6.2; DOI: 10.1186/s13578-025-01507-6
- Lycium barbarum and Lactobacillus acidophilus synergistically protect against anti-tuberculosis drug-induced male reproductive injury via gut microbiota-independent pathways in mice Frontiers in Microbiology; 2025; Impact Factor: 4.5; DOI: 10.3389/fmicb.2025.1622017
- Estimates of Natural Frequencies for Nuclear Vibration, and an Assessment of the Feasibility of Selective Ultrasound Ablation of Cancer Cells Journal of the Mechanical Behavior of Biomedical Materials; 2024; Impact Factor: 3.3; DOI: 10.1016/j.jmbbm.2024.106778
- Characterization of Proliferation Medium and Its Effect on Differentiation of Muscle Satellite Cells from Larimichthys crocea in Cultured Fish Meat Production Fishes; 2023; Impact Factor: 2.3; DOI: 10.3390/fishes8090429
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