PPID Recombinant monoclonal antibody 84115-6-RR proteintech
$149.00
In stock
SKU
84115-6-RR
| Catalog Number | 84115-6-RR |
| Synonyms | 241361E4, 40 kDa peptidyl-prolyl cis-trans isomerase, Cyclophilin 40, Cyclophilin D, Cyclophilin-40 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, FC (Intra), ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A549 cells, MCF-7 cells, Jurkat cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue |
| Tested Applications — Positive FC (Intra) detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | A549 cells, MCF-7 cells, Jurkat cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue |
| Positive FC (Intra) detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse, rat |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag3409 Product name: Recombinant human PPID protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 57-370 aa of BC030707 Sequence: GIGHTTGKPLHFKGCPFHRIIKKFMIQGGDFSNQNGTGGESIYGEKFEDENFHYKHDREGLLSMANAGRNTNGSQFFITTVPTPHLDGKHVVFGQVIKGIGVARILENVEVKGEKPAKLCVIAECGELKEGDDGGIFPKDGSGDSHPDFPEDADIDLKDVDKILLITEDLKNIGNTFFKSQNWEMAIKKYAEVLRYVDSSKAVIETADRAKLQPIALSCVLNIGACKLKMSNWQGAIDSCLEALELDPSNTKALYRRAQGWQGLKEYDQALADLKKAQGIAPEDKAIQAELLKVKQKIKAQKDKEKAVYAKMFA Predict reactive species |
| Full Name | peptidylprolyl isomerase D |
| Calculated Molecular Weight | 370 aa, 41 kDa |
| Observed Molecular Weight | 41 kDa |
| GenBank Accession Number | BC030707 |
| Gene Symbol | PPID |
| Gene ID (NCBI) | 5481 |
| RRID | AB_3671680 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q08752 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for PPID antibody 84115-6-RR | Download protocol |
| WB protocol for PPID antibody 84115-6-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 241361E4 |
| Conjugation | Unconjugated |
PPID(Peptidyl-prolyl cis-trans isomerase D) is also named CYP40, CYPD, and belongs to the cyclophilin-type PPIase family, which accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The inactivation of the Ppid gene rescued the disease phenotype of Col6a1 deficiency (PMID:19293339). This PPID antibody detected a strong band at an apparent molecular mass of 41 kDa and a weak band at 70 kDa due to crosslink (PMID: 33320090, 29097228). Protocols Product Specific Protocols FC protocol for PPID antibody 84115-6-RR Download protocol WB protocol for PPID antibody 84115-6-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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