Anti-GGGGS Linker Rabbit Recombinant Antibody 98262-1-RR proteintech
$229.00
In stock
SKU
98262-1-RR
| Catalog Number | 98262-1-RR |
| Synonyms | G4S, G4S link, G4S Linker, GGGGS, GS Linker |
| Host | Rabbit |
| Reactivity | recombinant protein |
| Form | Liquid |
| Formulation | PBS, Azide |
| Applications | WB, IHC, IF/ICC, FC |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | Transfected HEK-293T cells |
| Tested Applications — Positive IHC detected in | Transfected HEK-293T cells Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | Transfected HEK-293T cells, Transfected CHO-K1 |
| Tested Applications — Positive FC detected in | Transfected HEK-293T cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:6000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:40000-1:160000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:2000-1:8000 |
| Positive WB detected in | Transfected HEK-293T cells |
| Positive IHC detected in | Transfected HEK-293T cells Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Transfected HEK-293T cells, Transfected CHO-K1 |
| Positive FC detected in | Transfected HEK-293T cells |
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:40000-1:160000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:2000-1:8000 |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| Tested Reactivity | recombinant protein |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | GGGGS Linker |
| RRID | AB_3745928 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purfication |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
| FC protocol for GGGGS Linker antibody 98262-1-RR | Download protocol |
| IF protocol for GGGGS Linker antibody 98262-1-RR | Download protocol |
| IHC protocol for GGGGS Linker antibody 98262-1-RR | Download protocol |
| WB protocol for GGGGS Linker antibody 98262-1-RR | Download protocol |
| Daniela (Verified Customer) (10-27-2025) | The antibody was used as coating antibody for an ELISA setup (tested 1 µg/ml, 10 µg/ml)d: 1 µg/ml, 5 µ to quantify a protein containing a (GGGGS)4 motif (0.5 µg/ml followed by detection using a b2m-biotin antibody) which was not working at all. According to the tech support, detection of a (GGGGS)4 motif is not working, but it would be working good for a (GGGGS)3 motif which could not be tested due to lack of material. A 4GS antibody from CST and from Genscript were used as positive controls that were both working. Applications: Other Primary Antibody Dilution: 1 µg/ml, 5 µg/ml, 10 µg/ml Cell Tissue Type: MCH class 1 modified protein GGGGS Linker Antibody Other validation (1 µg/ml, 5 µg/ml, 10 µg/ml dilution) in MCH class 1 modified protein (Cat no:98262-1-RR) |
| Citations | 1 |
| Dilutions | WB : 1:1000-1:6000 IHC : 1:40000-1:160000 IF/ICC : 1:2000-1:8000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 242306C8 |
| Conjugation | Unconjugated |
As a crucial element in the design of recombinant fusion proteins, linkers play an increasingly vital role in the construction of stable, bioactive fusion proteins (PMID: 23026637). GGGGS linker (G4S linker) is a flexible linker made of 4 glycine repeats followed by a serine amino acid (PMID:3045807). Due to its flexibility and resistance to proteases, GGGGS and its repeats are commonly used when engineering a protein, particularly in the construction of single-chain Fv (ScFv) domains expressed on the surfaces of CAR-T cells (PMID:23581628; 36874404). This antibody, raised against a synthetic peptide (GGGGSGGGGSGGGGS), can recognize G4S*2, G4S*3, G4S*4, and G4S*5. Protocols Product Specific Protocols FC protocol for GGGGS Linker antibody 98262-1-RR Download protocol IF protocol for GGGGS Linker antibody 98262-1-RR Download protocol IHC protocol for GGGGS Linker antibody 98262-1-RR Download protocol WB protocol for GGGGS Linker antibody 98262-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Sucrose Isomerase Mutants' Expression in Bacillus subtilis for Isomaltulose Production.
Journal: Microorganisms | Authors: Xiaoyang Liu | Application: WB
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