DVL2 Polyclonal antibody 12037-1-AP proteintech
$149.00
In stock
SKU
12037-1-AP
| Catalog Number | 12037-1-AP |
| Synonyms | Dishevelled 2, Dishevelled-2, DSH homolog 2, Segment polarity protein dishevelled homolog DVL-2 |
| Host | Rabbit |
| Reactivity | human, mouse, rat and More (3) |
| Reactivity | human, mouse, rat, pig, chicken, xenopus |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, CoIP, ELISA |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293 cells, HepG2 cells, MCF-7 cells, MDA-MB-453s cells, mouse thymus tissue, MDA-MB-231 cells, NIH/3T3 cells |
| Tested Applications — Positive IP detected in | MCF-7 cells |
| Tested Applications — Positive IHC detected in | human lung cancer tissue, human gliomas tissue, human prostate cancer tissue, mouse colon tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:16000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | HEK-293 cells, HepG2 cells, MCF-7 cells, MDA-MB-453s cells, mouse thymus tissue, MDA-MB-231 cells, NIH/3T3 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human lung cancer tissue, human gliomas tissue, human prostate cancer tissue, mouse colon tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken, xenopus |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag2666 Product name: Recombinant human DVL2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-340 aa of BC014844 Sequence: MAGSSTGGGGVGETKVIYHLDEEETPYLVKIPVPAERITLGDFKSVLQRPAGAKYFFKSMDQDFGVVKEEISDDNARLPCFNGRVVSWLVSSDNPQPEMAPPVHEPRAELAPPAPPLPPLPPERTSGIGDSRPPSFHPNVSSSHENLEPETETESVVSLRRERPRRRDSSEHGAGGHRTGGPSRLERHLAGYESSSTLMTSELESTSLGDSDEEDTMSRFSSSTEQSSASRLLKRHRRRRKQRPPRLERTSSFSSVTDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDMNFENMSNDDAVRVLRDI Predict reactive species |
| Full Name | dishevelled, dsh homolog 2 (Drosophila) |
| Calculated Molecular Weight | 736 aa, 79 kDa |
| Observed Molecular Weight | 90-95 kDa |
| GenBank Accession Number | BC014844 |
| Gene Symbol | DVL2 |
| Gene ID (NCBI) | 1856 |
| RRID | AB_2093330 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14641 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for DVL2 antibody 12037-1-AP | Download protocol |
| IHC protocol for DVL2 antibody 12037-1-AP | Download protocol |
| IP protocol for DVL2 antibody 12037-1-AP | Download protocol |
| WB protocol for DVL2 antibody 12037-1-AP | Download protocol |
| mouse | IHC Front Cell Dev Biol Non-Canonical Wnt Signaling Regulates Cochlear Outgrowth and Planar Cell Polarity via Gsk3β Inhibition. Authors - Andre Landin Malt View Article |
| human | WB Development The phosphatase Pgam5 antagonizes Wnt/β-Catenin signaling in embryonic anterior-posterior axis patterning. Authors - Verena Rauschenberger View Article |
| Citations | 36 |
| Dilutions | WB : 1:2000-1:16000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
DVL2 is member of the dishevelled (dsh) protein family. DVL2 encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein in a variety of cell lines and species. DVL2 participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. It promotes internalization and degradation of frizzled proteins upon Wnt signaling. It plays a role both in canonical and non-canonical Wnt signaling. It also Plays a role in the signal transduction pathways mediated by multiple Wnt genes. Protocols Product Specific Protocols IF protocol for DVL2 antibody 12037-1-AP Download protocol IHC protocol for DVL2 antibody 12037-1-AP Download protocol IP protocol for DVL2 antibody 12037-1-AP Download protocol WB protocol for DVL2 antibody 12037-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Wnts regulate planar cell polarity via heterotrimeric G protein and PI3K signaling.
Journal: J Cell Biol | Authors: Andre Landin Malt | Species: mouse | Application: WB - Electroacupuncture promotes neurogenesis in the dentate gyrus and improves pattern separation in an early Alzheimer's disease mouse model
Journal: Biol Res | Authors: Yanyi Ding | Species: mouse | Application: WB - Regulation of the WNT-CTNNB1 signaling pathway by severe fever with thrombocytopenia syndrome virus in a cap-snatching manner
Journal: mBio | Authors: Xia-Ming Jiang | Species: human | Application: WB - YAP triggers the Wnt/β-catenin signalling pathway and promotes enterocyte self-renewal, regeneration and tumorigenesis after DSS-induced injury.
Journal: Cell Death Dis | Authors: Feihong Deng | Species: human | Application: WB - The phosphatase Pgam5 antagonizes Wnt/β-Catenin signaling in embryonic anterior-posterior axis patterning.
Journal: Development | Authors: Verena Rauschenberger | Species: human | Application: WB - Non-Canonical Wnt Signaling Regulates Cochlear Outgrowth and Planar Cell Polarity via Gsk3β Inhibition.
Journal: Front Cell Dev Biol | Authors: Andre Landin Malt | Species: mouse | Application: IHC
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