COA7 Polyclonal antibody 25361-1-AP proteintech
$149.00
In stock
SKU
25361-1-AP
| Catalog Number | 25361-1-AP |
| Synonyms | C1orf163, Beta-lactamase hcp-like protein, Cytochrome c oxidase assembly factor 7, RESA1, Respiratory chain assembly factor 1 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A431 cells, COLO 320 cells, PC-3 cells |
| Tested Applications — Positive IHC detected in | human placenta tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:5000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | A431 cells, COLO 320 cells, PC-3 cells |
| Positive IHC detected in | human placenta tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag19734 Product name: Recombinant human C1orf163 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-231 aa of BC015313 Sequence: MAGMVDFQDEEQVKSFLENMEVECNYHCYHEKDPDGCYRLVDYLEGIRKNFDEAAKVLKFNCEENQHSDSCYKLGAYYVTGKGGLTQDLKAAARCFLMACEKPGKKSIAACHNVGLLAHDGQVNEDGQPDLGKARDYYTRACDGGYTSSCFNLSAMFLQGAPGFPKDMDLACKYSMKACDLGHIWACANASRMYKLGDGVDKDEAKAEVLKNRAQQLHREQQKGVQPLTFG Predict reactive species |
| Full Name | chromosome 1 open reading frame 163 |
| Calculated Molecular Weight | 231 aa, 26 kDa |
| Observed Molecular Weight | 25-28 kDa |
| GenBank Accession Number | BC015313 |
| Gene Symbol | COA7 |
| Gene ID (NCBI) | 65260 |
| RRID | AB_2880043 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96BR5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for COA7 antibody 25361-1-AP | Download protocol |
| IHC protocol for COA7 antibody 25361-1-AP | Download protocol |
| WB protocol for COA7 antibody 25361-1-AP | Download protocol |
| human | WB,IF iScience Bisbiguanide analogs induce mitochondrial stress to inhibit lung cancer cell invasion Authors - Christina M Knippler View Article |
| mouse | WB Biogerontology Multi-omics profiling reveals mitochondrial dysfunction and epigenetic dysregulation in postoperative cognitive dysfunction: identification of novel biomarkers in an aged mouse model. Authors - Li Hu View Article |
| Citations | 4 |
| Dilutions | WB : 1:1000-1:5000 IHC : 1:500-1:2000 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Cytochrome c oxidase assembly factor 7 (COA7) is a 231 aa long mitochondrial protein present in animals, containing five Sel1-like tetratricopeptide repeat sequences, which are likely to interact with partner proteins (PMID: 30885959). COA7 is a metazoan-specific assembly factor, the absence or mutation of which in humans accompanies complex IV assembly defects and neurological conditions (PMID: 35210360). COA7 has a molecular weight of ~25 kDa. Protocols Product Specific Protocols IF protocol for COA7 antibody 25361-1-AP Download protocol IHC protocol for COA7 antibody 25361-1-AP Download protocol WB protocol for COA7 antibody 25361-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- COA7 (C1orf163/RESA1) mutations associated with mitochondrial leukoencephalopathy and cytochrome c oxidase deficiency.
Journal: J Med Genet | Authors: Anabel Martinez Lyons | Species: human | Application: WB - Discovery of bactericides as an acute mitochondrial membrane damage inducer.
Journal: Mol Biol Cell | Authors: Ryan Houston | Species: human | Application: WB - Multi-omics profiling reveals mitochondrial dysfunction and epigenetic dysregulation in postoperative cognitive dysfunction: identification of novel biomarkers in an aged mouse model.
Journal: Biogerontology | Authors: Li Hu | Species: mouse | Application: WB - Bisbiguanide analogs induce mitochondrial stress to inhibit lung cancer cell invasion
Journal: iScience | Authors: Christina M Knippler | Species: human | Application: WB,IF
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