REST Polyclonal antibody 22242-1-AP proteintech
$149.00
In stock
SKU
22242-1-AP
| Catalog Number | 22242-1-AP |
| Synonyms | NRSF, X2 box repressor, XBR |
| Host | Rabbit |
| Reactivity | human and More (2) |
| Reactivity | human, zebrafish, hamster |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, CoIP, ChIP, ELISA |
| Applications | WB, IHC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | Raji cells, HEK-293 cells |
| Tested Applications — Positive IP detected in | HeLa cells |
| Tested Applications — Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:3000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Positive WB detected in | Raji cells, HEK-293 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Tested Reactivity | human |
| Cited Reactivity | human, zebrafish, hamster |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag17707 Product name: Recombinant human REST protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 748-1097 aa of BC132859 Sequence: ELSPPMEVVQKEPVKIELSPPIEVVQKEPVQMELSPPMGVVQKEPAQREPPPPREPPLHMEPISKKPPLRKDKKEKSNMQSERARKEQVLIEVGLVPVKDSWLLKESVSTEDLSPPSPPLPKENLREEASGDQKLLNTGEGNKEAPLQKVGAEEADESLPGLAANINESTHISSSGQNLNTPEGETLNGKHQTDSIVCEMKMDTDQNTRENLTGINSTVEEPVSPMLPPSAVEEREAVSKTALASPPATMAANESQEIDEDEGIHSHEGSDLSDNMSEGSDDSGLHGARPVPQESSRKNAKEALAVKAAKGDFVCIFCDRSFRKGKDYSKHLNRHLVNVYYLEEAAQGQE Predict reactive species |
| Full Name | RE1-silencing transcription factor |
| Calculated Molecular Weight | 1097 aa, 122 kDa |
| Observed Molecular Weight | 200 kDa |
| GenBank Accession Number | BC132859 |
| Gene Symbol | REST |
| Gene ID (NCBI) | 5978 |
| RRID | AB_2879044 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13127 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for REST antibody 22242-1-AP | Download protocol |
| IP protocol for REST antibody 22242-1-AP | Download protocol |
| WB protocol for REST antibody 22242-1-AP | Download protocol |
| mouse,human | WB,IP,IHC,IF Adv Sci (Weinh) Hepatocyte BDNF Acts as a Novel Immune Checkpoint to Restrain TLR4-Mediated Acute Hepatitis. Authors - Weiwei Zhu View Article |
| human | WB Cell Death Dis OPRK1 drives SLC9A3R1 progression to neuroendocrine prostate cancer. Authors - Linghui Liang View Article |
| Aktan (Verified Customer) (12-24-2019) | The product yield satisfactory results with 5%BSA in PBST blocking; primary dilution in the blocking buffer and o/n incubation. The blot is washed and incubated with Licor near IR dye-conjugated goat-antirabbit secondary antibodies for 1h and after washing the bands were detected in Licor. Applications: Western Blot, Primary Antibody Dilution: 1:500 Cell Tissue Type: Prostate cancer cell lines REST Antibody Western Blot, validation (1:500 dilution) in Prostate cancer cell lines (Cat no:22242-1-AP) |
| Citations | 42 |
| Dilutions | WB : 1:500-1:3000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:400-1:1600 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
REST also named as NRSF or X2 box repressor is a 1097 amino acid protein, which contains 9 C2H2-type zinc fingers. REST as transcriptional repressor binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Repressor element 1 (RE1) silencing transcription factor (REST), is widely expressed during embryogenesis, and plays a strategic role in terminal neuronal differentiation. The molecular weight of REST is 121 kDa, but REST was detected 200kDa by western blot in our result, which is similar to the one article (PMID: 16764822). Protocols Product Specific Protocols IHC protocol for REST antibody 22242-1-AP Download protocol IP protocol for REST antibody 22242-1-AP Download protocol WB protocol for REST antibody 22242-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Hepatocyte BDNF Acts as a Novel Immune Checkpoint to Restrain TLR4-Mediated Acute Hepatitis.
Journal: Adv Sci (Weinh) | Authors: Weiwei Zhu | Species: mouse,human | Application: WB,IP,IHC,IF - YAP drives fate conversion and chemoresistance of small cell lung cancer.
Journal: Sci Adv | Authors: Qingzhe Wu KD Validated - EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency
Journal: Sci Adv | Authors: Chikako Okubo | Species: human | Application: WB - The cross talk of two family members of β-TrCP in the regulation of cell autophagy and growth.
Journal: Cell Death Differ | Authors: Danrui Cui | Species: human | Application: WB - Prolactin-Releasing Hormone Receptor (PRLHR) enhances radiosensitivity and exacerbates DNA damage in glioblastoma post-irradiation by inhibiting Y-box-binding protein-1 (YBX1) nuclear translocation: a novel perspective on precision radiotherapy.
Journal: Mol Biomed | Authors: Yuning Qiu | Species: human | Application: IHC - OPRK1 drives SLC9A3R1 progression to neuroendocrine prostate cancer.
Journal: Cell Death Dis | Authors: Linghui Liang | Species: human | Application: WB - Loss of RE-1 silencing transcription factor accelerates exocrine damage from pancreatic injury.
Journal: Cell Death Dis | Authors: Julie K Bray | Application: WB - OPRK1 drives SLC9A3R1 progression to neuroendocrine prostate cancer.
Journal: Cell Death Dis | Authors: Linghui Liang | Species: human | Application: WB - KIF15 promotes human glioblastoma progression under the synergistic transactivation of REST and P300
Journal: Int J Biol Sci | Authors: Wendan Yu | Species: human | Application: WB,IP,IHC - Super-silencer perturbation by EZH2 and REST inhibition leads to large loss of chromatin interactions and reduction in cancer growth
Journal: Nat Struct Mol Biol | Authors: Ying Zhang | Species: human | Application: WB - Prioritization of risk genes in colorectal cancer by integrative analysis of multi-omics data and gene networks
Journal: Sci China Life Sci | Authors: Ming Zhang | Species: human | Application: ChIP - Crosstalk between cancer-associated fibroblasts and non-neuroendocrine tumor cells in small cell lung cancer involves in glycolysis and antigen-presenting features
Journal: Mol Med | Authors: Yuanhua Lu | Species: human | Application: IHC - Novel REST Truncation Mutations Causing Hereditary Gingival Fibromatosis.
Journal: J Dent Res | Authors: J T Chen | Species: human | Application: IHC - The role of REST in regulating the BDNF/TrkB signalling pathway in nano-alumina induced cognitive dysfunction in zebrafish
Journal: Ecotoxicol Environ Saf | Authors: Jingsi Zhang | Species: zebrafish | Application: WB - REST alleviates neurotoxic prion peptide-induced synaptic abnormalities, neurofibrillary degeneration and neuronal death partially via LRP6-mediated Wnt-β-catenin signaling.
Journal: Oncotarget | Authors: Zhiqi Song | Application: WB,IF - Epigenetic regulation of GABA catabolism in iPSC-derived neurons: The molecular links between FGF21 and histone methylation
Journal: Mol Metab | Authors: Ming-Fen Ho | Species: human | Application: WB,CoIP,ChIP - Functional Analysis of RE1 Silencing Transcription Factor as a Putative Tumor Suppressor in Human Endometrial Cancer
Journal: Int J Mol Sci | Authors: Yasmin Abedin | Species: human | Application: WB,IHC - HCN2 Promotes BGN Transcription via REST to Regulate Ferroptosis and Tumor Progression in Bladder Cancer.
Journal: Int J Mol Sci | Authors: Yudong Cao | Species: human | Application: ChIP - Inhibition of the Epigenetic Regulator REST Ameliorates Ischemic Brain Injury.
Journal: Mol Neurobiol | Authors: Kahlilia C Morris-Blanco | Species: mouse | Application: IF - Targeting of REST with rationally-designed small molecule compounds exhibits synergetic therapeutic potential in human glioblastoma cells
Journal: BMC Biol | Authors: Svetlana B Panina | Species: human | Application: WB - Anlotinib + Toripalimab maintenance in Extensive-Stage small cell lung Cancer: Clinical efficacy and preclinical mechanism of anlotinib-induced neuroendocrine differentiation Suppression via Notch1.
Journal: Lung Cancer | Authors: Dongqing Lv | Species: human | Application: WB - Regulation of nuclear transport of the transcriptional factor REST improves axon regeneration in peripheral nerves.
Journal: Mol Med Rep | Authors: Takamaru Suzuki | Species: mouse | Application: WB,IF - Glycoprotein 130 improves repressor element‑1 silencing transcription factor‑related axon regenerative capacity in peripheral nerves with aging
Journal: Mol Med Rep | Authors: So Kawakita | Species: mouse | Application: WB,IF - Remodeling of the m6A landscape in the heart reveals few conserved post-transcriptional events underlying cardiomyocyte hypertrophy.
Journal: J Mol Cell Cardiol | Authors: Scott A Hinger | Species: human | Application: WB - Dysregulation of REST and its target genes impacts the fate of neural progenitor cells in down syndrome
Journal: Sci Rep | Authors: Tan Huang | Application: WB - EPO modified MSCs protects SH-SY5Y cells against ischemia/hypoxia-induced apoptosis via REST-dependent epigenetic remodeling
Journal: Sci Rep | Authors: Yu Jiang | Species: human | Application: WB - Small intestinal neuroendocrine tumors lack early genomic drivers, acquire DNA repair defects and harbor hallmarks of low REST expression
Journal: Sci Rep | Authors: Felix Bolduan | Species: human | Application: WB - Dynamic phenotypic reprogramming and chemoresistance induced by lung fibroblasts in small cell lung cancer
Journal: Sci Rep | Authors: Yuanhua Lu | Species: human | Application: IHC - Systematic analysis identifies REST as an oncogenic and immunological biomarker in glioma
Journal: Sci Rep | Authors: Guan Wang | Species: human | Application: WB,IHC - Lithium alleviates neurotoxic prion peptide-induced synaptic damage and neuronal death partially by the upregulation of nuclear target REST and the restoration of Wnt signaling.
Journal: Neuropharmacology | Authors: Zhiqi Song | Application: WB,IF - Silybin ameliorates Pb-induced learning and memory impairment by inhibiting STAT3/hnRNP U/REST and NFκB/hnRNP U.
Journal: Environ Toxicol Pharmacol | Authors: Wei Zhang | Application: WB - NLRP3 Inflammasome Activation Enhances ADK Expression to Accelerate Epilepsy in Mice
Journal: Neurochem Res | Authors: Haiju Zhang | Species: mouse | Application: WB - Downregulation of the Repressor Element 1-Silencing Transcription Factor (REST) Is Associated with Akt-mTOR and Wnt-β-Catenin Signaling in Prion Diseases Models.
Journal: Front Mol Neurosci | Authors: Zhiqi Song | Species: hamster | Application: WB,IF - The restoration of REST inhibits reactivity of Down syndrome iPSC-derived astrocytes
Journal: Front Mol Neurosci | Authors: Tan Huang | Species: human | Application: IF - LRRC8D Suppresses Prostate Cancer Growth and Enhances Platinum Sensitivity via Modulation of CAV-1/STAT3 Signaling.
Journal: Membranes (Basel) | Authors: Rong Xu - DNMT3b-mediated CpA methylation facilitates REST binding and gene silencing and exacerbates hippocampal demyelination in diabetic mice
Journal: J Biol Chem | Authors: Tie-Feng Yao | Species: mouse | Application: WB,IHC - REST deficiency and neurogenic-to-gliogenic shift in down syndrome human cerebral organoids.
Journal: Mol Brain | Authors: Tan Huang | Species: human | Application: IF - Comprehensive Analysis of REST/NRSF Gene in Glioma and Its ceRNA Network Identification.
Journal: Front Med (Lausanne) | Authors: Yulian Zhang | Species: human | Application: WB - Overexpression of REST Causes Neuronal Injury and Decreases Cofilin Phosphorylation in Mice
Journal: J Alzheimers Dis | Authors: Xiang Liu | Species: mouse | Application: WB,IHC,IF - Age‑related differences for expression of the nerve‑specific proteins after peripheral nerve injury
Journal: Exp Ther Med | Authors: Hiroyuki Obata | Species: mouse | Application: IF - Repressor element 1-silencing transcription factor deficiency yields profound hearing loss through Kv7.4 channel upsurge in auditory neurons and hair cells
Journal: Elife | Authors: Haiwei Zhang | Species: mouse | Application: IF - RE-1 silencing transcription factor is reduced in endometriosis and uterine deletion in mice alters progesterone responsiveness.
Journal: bioRxiv | Authors: Paige Minchella | Species: human | Application: IHC
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