| Catalog Number | CL488-15197 |
| Synonyms | CA III, CA3, CAIII, Car3, Carbonate dehydratase III, Carbonic anhydrase 3, Carbonic anhydrase III |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Proclin300, BSA, Glycerol |
| Applications | IF-P |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive IF-P detected in | mouse skeletal muscle tissue |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Positive IF-P detected in | mouse skeletal muscle tissue |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag7344 Product name: Recombinant human CA3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-260 aa of BC004897 Sequence: MAKEWGYASHNGPDHWHELFPNAKGENQSPIELHTKDIRHDPSLQPWSVSYDGGSAKTILNNGKTCRVVFDDTYDRSMLRGGPLPGPYRLRQFHLHWGSSDDHGSEHTVDGVKYAAELHLVHWNPKYNTFKEALKQRDGIAVIGIFLKIGHENGEFQIFLDALDKIKTKGKEAPFTKFDPSCLFPACRDYWTYQGSFTTPPCEECIVWLLLKEPMTVSSDQMAKLRSLLSSAENEPPVPLVSNWRPPQPINNRVVRASFK Predict reactive species |
| Full Name | carbonic anhydrase III, muscle specific |
| Calculated Molecular Weight | 29 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC004897 |
| Gene Symbol | CA3 |
| Gene ID (NCBI) | 761 |
| RRID | AB_3672623 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07451 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. |
| IF protocol for CL Plus 488 CA3 antibody CL488-15197 | Download protocol |
| Citations | - |
| Dilutions | IF-P : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | CoraLite® Plus 488 |
Carbonic anhydrase III (CA3), which belongs to the alpha-carbonic anhydrase family, is a cytoplasmic enzyme that exhibits a relatively low carbon dioxide hydratase activity. It is expressed at a very high level in skeletal muscle, where physical exercise has been shown to increase free radical production. In addition to its carbon dioxide hydratase activity, CA3 has been demonstrated to have a carboxyl esterase activity and phosphatase activity, which suggests that it is a tyrosine phosphatase (PMID: 10064618). CA3 was found to be localized in Type-I muscle fibers and could be used as a marker for abnormal Type-I muscle fibers in several neuromuscular diseases (PMID: 6221502). Protocols Product Specific Protocols IF protocol for CL Plus 488 CA3 antibody CL488-15197 Download protocol Standard Protocols Click here to view our Standard Protocols
Product Documents