Angiotensinogen/AGT Polyclonal antibody 11992-1-AP proteintech
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SKU
11992-1-AP
| Catalog Number | 11992-1-AP |
| Synonyms | AGT, Angiotensinogen, Serpin A8, SERPINA8, Alpha 1 antiproteinase |
| Host | Rabbit |
| Reactivity | human and More (2) |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA |
| Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HuH-7 cells, HepG2 cells, human plasma |
| Tested Applications — Positive IP detected in | human plasma |
| Tested Applications — Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Tested Applications — Positive FC (Intra) detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | HuH-7 cells, HepG2 cells, human plasma |
| Positive IP detected in | human plasma |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag2632 Product name: Recombinant human AGT protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 136-485 aa of BC011519 Sequence: PTAVFGTLASLYLGALDHTADRLQAILGVPWKDKNCTSRLDAHKVLSALQAVQGLLVAQGRADSQAQLLLSTVVGVFTAPGLHLKQPFVQGLALYTPVVLPRSLDFTELDVAAEKIDRFMQAVTGWKTGCSLTGASVDSTLAFNTYVHFQGKMKGFSLLAEPQEFWVDNSTSVSVPMLSGMGTFQHWSDIQDNFSVTQVSFTESACLLLIQPHYASDLDKVEGLTFQQNSLNWMKKLSPRTIHLTMPQLVLQGSYDLQDLLAQAELPAILHTELNLQKLSNDRIRVGEVLNSIFFELEADEREPTESTQQLNKPEVLEVTLNRPFLFAVYDQSATALHFLGRVANPLSTA Predict reactive species |
| Full Name | angiotensinogen (serpin peptidase inhibitor, clade A, member 8) |
| Calculated Molecular Weight | 485 aa, 53 kDa |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | BC011519 |
| Gene Symbol | AGT |
| Gene ID (NCBI) | 183 |
| RRID | AB_2225452 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P01019 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for Angiotensinogen/AGT antibody 11992-1-AP | Download protocol |
| IF protocol for Angiotensinogen/AGT antibody 11992-1-AP | Download protocol |
| IHC protocol for Angiotensinogen/AGT antibody 11992-1-AP | Download protocol |
| IP protocol for Angiotensinogen/AGT antibody 11992-1-AP | Download protocol |
| WB protocol for Angiotensinogen/AGT antibody 11992-1-AP | Download protocol |
| human | WB Cells Effect of Hydrocortisone on Angiotensinogen (AGT) Mutation-Causing Autosomal Recessive Renal Tubular Dysgenesis. Authors - Min-Hua Tseng View Article |
| mouse | IHC,IF FASEB J Sperm immotility is associated with epididymis metabolism disorder in mice under obstructive azoospermia Authors - Sheng Gao View Article |
| Citations | 32 |
| Dilutions | WB : 1:1000-1:4000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:50-1:500 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Angiotensinogen is a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene also have been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease. Protocols Product Specific Protocols FC protocol for Angiotensinogen/AGT antibody 11992-1-AP Download protocol IF protocol for Angiotensinogen/AGT antibody 11992-1-AP Download protocol IHC protocol for Angiotensinogen/AGT antibody 11992-1-AP Download protocol IP protocol for Angiotensinogen/AGT antibody 11992-1-AP Download protocol WB protocol for Angiotensinogen/AGT antibody 11992-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- KDM4A-induced tumor senescence enhances the efficacy of immunotherapy by inhibiting AGT-PHB1 axis-mediated mitophagy in colorectal cancer
Journal: Autophagy | Authors: Tanxing Cai - Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.
Journal: Matrix Biol | Authors: Emna Ouni | Species: human | Application: IF - Attenuation of brain damage and cognitive impairment by direct renin inhibition in mice with chronic cerebral hypoperfusion.
Journal: Hypertension | Authors: Dong Yi-Fei YF | Species: mouse | Application: IHC - Downregulation of spinal angiotensin converting enzyme 2 is involved in neuropathic pain associated with type 2 diabetes mellitus in mice.
Journal: Biochem Pharmacol | Authors: Ryota Yamagata | Species: mouse | Application: WB - Sperm immotility is associated with epididymis metabolism disorder in mice under obstructive azoospermia
Journal: FASEB J | Authors: Sheng Gao | Species: mouse | Application: IHC,IF - Effect of Hydrocortisone on Angiotensinogen (AGT) Mutation-Causing Autosomal Recessive Renal Tubular Dysgenesis.
Journal: Cells | Authors: Min-Hua Tseng | Species: human | Application: WB - Heshuxiaoji pill suppresses steatohepatitis and fibrosis by regulating the AngII-BACH1 mediated vasoconstriction
Journal: J Ethnopharmacol | Authors: Yueheng Pu | Species: human | Application: WB,IF - A novel stroke rehabilitation strategy and underlying stress granule regulations through inhibition of NLRP3 inflammasome activation
Journal: CNS Neurosci Ther | Authors: Qingzhu Wang | Species: rat | Application: WB - Antihyperalgesic effects of ACE2 activation in a neuropathic pain model involve spinal MAS1 receptor signaling and ATP-sensitive potassium channels.
Journal: Biochem Pharmacol | Authors: Ribou Shiga - Downregulation of spinal angiotensin converting enzyme 2 is involved in neuropathic pain associated with type 2 diabetes mellitus in mice.
Journal: Biochem Pharmacol | Authors: Ryota Yamagata | Species: mouse | Application: WB - Effect of Hydrocortisone on Angiotensinogen (AGT) Mutation-Causing Autosomal Recessive Renal Tubular Dysgenesis.
Journal: Cells | Authors: Min-Hua Tseng | Species: human | Application: WB - RecQ dysfunction contributes to social and depressive-like behavior and affects aldolase activity in mice
Journal: Neurobiol Dis | Authors: Yujun Hou | Species: mouse | Application: WB - Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.
Journal: Matrix Biol | Authors: Emna Ouni | Species: human | Application: IF - Modulation of the local angiotensin II: Augmentation of ferroptosis and radiosensitivity in nasopharyngeal carcinoma via the HIF-1α-HILPDA axis
Journal: Radiother Oncol | Authors: Xiuting Huang | Species: human | Application: WB,IHC,CoIP - Expression of Renin-Angiotensin System Components in the Taste Organ of Mice.
Journal: Nutrients | Authors: Noriatsu Shigemura | Species: mouse | Application: IHC - Glycyrrhizic acid restores the downregulated hepatic ACE2 signaling in the attenuation of mouse steatohepatitis
Journal: Eur J Pharmacol | Authors: Longyue Zhou | Species: mouse | Application: WB - Protective effect of recombinant Lactobacillus plantarum against H2O2-induced oxidative stress in HUVEC cells.
Journal: J Zhejiang Univ Sci B | Authors: Guan Wang | Species: human | Application: WB - AGT serves as a potential biomarker and drives tumor progression in colorectal carcinoma.
Journal: Int Immunopharmacol | Authors: Wei Chen | Species: human | Application: WB,IHC - Fuyuan Xingnao Decoction Promotes Angiogenesis Through the Rab1/AT1R Pathway in Diabetes Mellitus Complicated With Cerebral Infarction.
Journal: Front Pharmacol | Authors: Dong Deng | Species: rat | Application: WB - Astragaloside IV protects against podocyte injury via SERCA2-dependent ER stress reduction and AMPKα-regulated autophagy induction in streptozotocin-induced diabetic nephropathy.
Journal: Sci Rep | Authors: Hengjiang Guo | Species: mouse | Application: WB - Sperm immotility is associated with epididymis metabolism disorder in mice under obstructive azoospermia
Journal: FASEB J | Authors: Sheng Gao | Species: mouse | Application: IHC,IF - Novel fusion protein PK5-RL-Gal-3C inhibits hepatocellular carcinoma via anti-angiogenesis and cytotoxicity
Journal: BMC Cancer | Authors: Xiaoge Gao | Species: human | Application: WB - Adequate salt intake is essential for candesartan-treated rats to maintain renal function
Journal: Am J Physiol Renal Physiol | Authors: Anni Xie | Species: rat | Application: WB - Retrospective studies and quantitative proteomics reveal that abnormal expression of blood pressure, blood lipids, and coagulation related proteins is associated with hypospadias
Journal: Hum Genet | Authors: Kexin Zhang | Species: human | Application: IHC - ALDH2 protects against alcoholic cardiomyopathy through a mechanism involving the p38 MAPK/CREB pathway and local renin-angiotensin system inhibition in cardiomyocytes.
Journal: Int J Cardiol | Authors: Baoshan Liu | Species: mouse | Application: WB - Involvement of the spinal angiotensin II system in streptozotocin-induced diabetic neuropathic pain in mice.
Journal: Mol Pharmacol | Authors: Yoshiki Ogata | Species: mouse | Application: WB - Intrarenal renin-angiotensin system activation and macrophage infiltrations in pediatric chronic glomerulonephritis
Journal: Pediatr Nephrol | Authors: Tomoki Hattori | Species: human | Application: IHC,IF - Mechanical stretch induces angiotensinogen expression through PARP1 activation in kidney proximal tubular cells.
Journal: In Vitro Cell Dev Biol Anim | Authors: Jeong Soon Lee | Species: mouse | Application: WB - The Key Role of Initiation Timing on Stroke Rehabilitation by Remote Ischemic Conditioning with Exercise (RICE)
Journal: Neurol Res | Authors: Qingzhu Wang | Species: rat | Application: WB - Autophagy-enabled protein degradation: Key to platelet activation and ANGII production in patients with type 2 diabetes mellitus
Journal: Heliyon | Authors: Qiang Wu | Species: mouse | Application: WB - Autophagy-enabled protein degradation: Key to platelet activation and ANGII production in patients with type 2 diabetes mellitus
Journal: Heliyon | Authors: Qiang Wu | Species: mouse | Application: WB - Cell-cell interaction determines cell fate of mesoderm-derived cell in tongue development through Hh signaling
Journal: Elife | Authors: Maiko Kawasaki | Species: mouse | Application: IF
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