Phospho-YAP1 (Ser397) Polyclonal antibody 29018-1-AP proteintech
$149.00
In stock
SKU
29018-1-AP
| Catalog Number | 29018-1-AP |
| Synonyms | p YAP1, p YAP1 (Ser397), p YAP1 Ser397, Phospho YAP (Ser397), Phospho YAP1 |
| Host | Rabbit |
| Reactivity | human, mouse and More (1) |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, ELISA |
| Applications | WB, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | Serum-free treated NIH/3T3 cells, 10 % fetal bovine serum treated NIH/3T3 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:16000 |
| Positive WB detected in | Serum-free treated NIH/3T3 cells, 10 % fetal bovine serum treated NIH/3T3 cells |
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | Yes-associated protein 1, 65kDa |
| Calculated Molecular Weight | 504 aa, 54 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC038235 |
| Gene Symbol | YAP1 |
| Gene ID (NCBI) | 10413 |
| RRID | AB_2923587 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P46937 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| WB protocol for Phospho-YAP1 (Ser397) antibody 29018-1-AP | Download protocol |
| human | WB Front Immunol The role of YAP1 in survival prediction, immune modulation, and drug response: A pan-cancer perspective Authors - Xueqing Hu View Article |
| rat,mouse | WB Int Immunopharmacol ASIC1a regulates ferroptosis in hepatic stellate cells via the Hippo/Yap-1 pathway in liver fibrosis Authors - Yueqin Zhu View Article |
| mouse | WB Int Immunopharmacol Ischemia-inhibited ferric chelate reductase 1 improves ferroptosis-mediated intestinal ischemia injury via Hippo signaling Authors - Pengzhen Liang View Article |
| Udesh (Verified Customer) (08-19-2024) | Worked well to detect pYAP at 70 kDa Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: HEK293T Phospho-YAP1 (Ser397) Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:29018-1-AP) |
| Citations | 54 |
| Dilutions | WB : 1:2000-1:16000 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
The transcriptional factors Yes-associated protein 1 (YAP1) and PDZ-binding motif (TAZ) act as downstream effectors of the Hippo pathway, and their subcellular location and transcriptional activities are affected by multiple post-translational modifications (PTMs). After phosphorylation, YAP1/TAZ binds to the 14-3-3 protein, which induces cytoplasmic retention of YAP1/TAZ. Moreover, phosphorylated YAP1/TAZ is likely to undergo ubiquitination degradation that is dependent on the β-transducin repeat-containing E3 ubiquitin protein ligase complex. The phosphorylation of YAP at Ser127 promotes its cytoplasmic retention, whereas phosphorylation at Ser397 induces degradation. (PMID: 29682330, PMID: 26039999) Protocols Product Specific Protocols WB protocol for Phospho-YAP1 (Ser397) antibody 29018-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation
Journal: Dev Cell | Authors: Wanxin Guo | Species: human | Application: WB - The role of YAP1 in survival prediction, immune modulation, and drug response: A pan-cancer perspective
Journal: Front Immunol | Authors: Xueqing Hu | Species: human | Application: WB - Microcystin-LR facilitates pubertal bone retardation by interfering with glutamine metabolism to accelerate stem cell senescence through Hippo/GLS pathway
Journal: Environ Pollut | Authors: Chun Pan | Application: WB - ASIC1a regulates ferroptosis in hepatic stellate cells via the Hippo/Yap-1 pathway in liver fibrosis
Journal: Int Immunopharmacol | Authors: Yueqin Zhu | Species: rat,mouse | Application: WB - Ischemia-inhibited ferric chelate reductase 1 improves ferroptosis-mediated intestinal ischemia injury via Hippo signaling
Journal: Int Immunopharmacol | Authors: Pengzhen Liang | Species: mouse | Application: WB - Daidzein alleviates CCl4- and BDL-induced liver fibrosis via suppressing the integrin alphaVbeta1/YAP signaling
Journal: Food Funct | Authors: Junjie Bai | Application: WB - PLAGL2 promotes bladder cancer progression via RACGAP1/RhoA GTPase/YAP1 signaling
Journal: Cell Death Dis | Authors: Hualin Chen | Species: human | Application: WB,IHC - Usenamine a potentiates anti-CRC activity of sorafenib by inducing autophagy and inhibiting YAP pathway through targeting SOD2.
Journal: Phytomedicine | Authors: Weishan Zhuge | Species: human | Application: WB - Yifei Sanjie pill combined with gefitinib reduces the progression of EGFR-TKIs-resistant non-small cell lung cancer via YAP/ANKRD1 axis.
Journal: Phytomedicine | Authors: Yi Yuan | Species: human | Application: WB - Synergistic Intervention of Piezo1 Activation and Fibrous Tissue Remodeling to Ease Side Effects of Intralesional Therapy for Keloids.
Journal: Adv Healthc Mater | Authors: Zixi Jiang | Species: human | Application: IF - Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation
Journal: Dev Cell | Authors: Wanxin Guo | Species: human | Application: WB - Piezo1 promotes intervertebral disc degeneration through the Ca2+/F-actin/Yap signaling axis
Journal: Mol Med | Authors: Fushuai Peng | Species: mouse | Application: WB - NCAPH-YAP1 interaction promotes breast cancer stemness and tumor progression
Journal: Stem Cell Res Ther | Authors: Caixin Qiu | Application: WB - Microcystin-LR facilitates pubertal bone retardation by interfering with glutamine metabolism to accelerate stem cell senescence through Hippo/GLS pathway
Journal: Environ Pollut | Authors: Chun Pan | Application: WB - The role of YAP1 in survival prediction, immune modulation, and drug response: A pan-cancer perspective
Journal: Front Immunol | Authors: Xueqing Hu | Species: human | Application: WB - Fusobacterium nucleatum increases CTGF expression through TLR2-YAP signaling axis in cancer-associated fibroblasts, thereby promoting colorectal cancer progression.
Journal: Cancer Cell Int | Authors: Haoran Wang | Species: human | Application: WB - Jiajian Shuyu Pills ameliorates cerebral ischemia-reperfusion injury by regulation hippo signaling and the lipid metabolism-ferroptosis axis.
Journal: J Ethnopharmacol | Authors: Xuecheng Yu - 3D pancreatic ductal adenocarcinoma desmoplastic model: Glycolysis facilitating stemness via ITGAV-PI3K-AKT-YAP1
Journal: Biomater Adv | Authors: Xiaoqi Guan | Species: human | Application: WB - Elucidating the correlation between polychlorinated dibenzo-p-dioxins and prostate cancer progression: Insights from gene expression and molecular docking
Journal: Ecotoxicol Environ Saf | Authors: Fei Lin | Species: human | Application: WB - Daidzein alleviates CCl4- and BDL-induced liver fibrosis via suppressing the integrin alphaVbeta1/YAP signaling
Journal: Food Funct | Authors: Junjie Bai | Application: WB - Leonurine Attenuates CCl4-Induced Hepatic Fibrosis in Mice via the Hippo-YAP Pathway
Journal: Drug Des Devel Ther | Authors: Pengru Wang | Species: human | Application: WB - High viscosity microenvironment induces chemoresistance of cancer cells through upregulating P-gp.
Journal: Int J Pharm | Authors: Guanlin Zhou | Species: human | Application: WB - Forskolin alleviates cholestatic liver disease by inhibiting the Hippo/YAP-mediated ductular reaction and fibrosis progression
Journal: Eur J Pharmacol | Authors: Walaa H El-Maadawy - Ginsenoside Rh2 inhibits epithelial-mesenchymal transition in triple-negative breast cancer cell via disrupting the HIF-1α/YAP nuclear translocation axis.
Journal: Int Immunopharmacol | Authors: Huijuan Liu | Species: human | Application: WB,IF - Hypoxia-induced tumor cell-intrinsic PLAU activation drives immunotherapy resistance in collagenic lung adenocarcinoma
Journal: Int Immunopharmacol | Authors: Li Wan | Species: human | Application: WB - Ginsenoside Rh2 inhibits epithelial-mesenchymal transition in triple-negative breast cancer cell via disrupting the HIF-1α/YAP nuclear translocation axis.
Journal: Int Immunopharmacol | Authors: Huijuan Liu | Species: human | Application: WB,IF - ASIC1a regulates ferroptosis in hepatic stellate cells via the Hippo/Yap-1 pathway in liver fibrosis
Journal: Int Immunopharmacol | Authors: Yueqin Zhu | Species: rat,mouse | Application: WB - Ischemia-inhibited ferric chelate reductase 1 improves ferroptosis-mediated intestinal ischemia injury via Hippo signaling
Journal: Int Immunopharmacol | Authors: Pengzhen Liang | Species: mouse | Application: WB - Taurine Attenuates Neuronal Ferroptosis by CSF-Derived Exosomes of GABABR Encephalitis Through GABABR/NF2/P-YAP Pathway
Journal: Mol Neurobiol | Authors: Chong Zhang | Species: human | Application: WB - New daphnane diterpenoidal 1,3,4-oxdiazole derivatives as potential anti-hepatoma agents: Synthesis, biological evaluation and molecular modeling studies
Journal: Bioorg Chem | Authors: Xinyi Wang | Species: human | Application: WB - Gingival proteomics reveals the role of TGF beta and YAP/TAZ signaling in Raine syndrome fibrosis
Journal: Sci Rep | Authors: Cláudio Rodrigues Rezende Costa | Species: human | Application: WB - Ginsenoside Rg1 ameliorates lipopolysaccharide-induced depressive-like behaviors in mice by attenuating neuroinflammation and neuronal damage.
Journal: Sci Rep | Authors: Lan Zhang | Species: mouse | Application: WB - DDR1 Targeting HOXA6 Facilitates Bladder Cancer Progression via Inhibiting Ferroptosis
Journal: J Cell Mol Med | Authors: Xin Xie | Species: human | Application: WB,IHC - PPP1CA promotes hepatocellular carcinoma progression in a YAP1-dependent way
Journal: Cell Signal | Authors: Xing Liu | Species: human | Application: WB - RNF144A-AS1 stabilizes TAF15 and promotes malignant biological behaviors of skin cutaneous melanoma
Journal: Mol Cell Biochem | Authors: Shude Yang | Species: human | Application: WB - Hippo/YAP1 promotes osteoporotic mice bone defect repair via the activating of Wnt signaling pathway
Journal: Cell Signal | Authors: Kehan Li | Application: WB - Silencing of fibronectin type III domain-containing protein 3A (FNDC3A) attenuates epithelial-to-mesenchymal transition (EMT), cancer invasion, and stemness in triple-negative breast cancer (TNBC)
Journal: Biochim Biophys Acta Mol Cell Res | Authors: Nanaka Wada - Macrophage-derived FN1 promotes peritoneal cavity metastasis of gastric cancer by inhibiting the Hippo signaling pathway via SDC4.
Journal: Biochim Biophys Acta Mol Cell Res | Authors: Meixia Zhang | Species: human | Application: WB,IHC - Connexin 43 dephosphorylation mediates the Dchs1/YAP/TEAD signaling pathway to induce cardiac fibrosis
Journal: Biochim Biophys Acta Mol Cell Res | Authors: Min Wang | Species: rat | Application: WB - Oridonin suppresses the growth of glioblastoma cells via inhibiting Hippo/YAP axis
Journal: Arch Biochem Biophys | Authors: Chen Wang | Species: mouse | Application: WB - The MIR181A2HG/miR-5680/VCAN-CD44 Axis Regulates Gastric Cancer Lymph Node Metastasis by Promoting M2 Macrophage Polarization
Journal: Cancer Med | Authors: Weijie Zang | Species: human | Application: WB - Mechanical stretch promotes the migration of mesenchymal stem cells via Piezo1/F-actin/YAP axis
Journal: Exp Cell Res | Authors: Ning Ma | Species: rat | Application: WB - LATS-regulated nuclear-cytoplasmic translocation of SREBP2 inhibits hepatocellular carcinoma cell migration and invasion via epithelial-mesenchymal transition
Journal: Mol Carcinog | Authors: Feng Zhang | Species: human | Application: WB - FATP5 modulates biological activity and lipid metabolism in prostate cancer through the TEAD4-mediated Hippo signaling
Journal: Front Oncol | Authors: Shenyang Liu | Species: human | Application: WB - Targeting mechanics-induced trabecular meshwork dysfunction through YAP-TGFβ Ameliorates high myopia-induced ocular hypertension
Journal: Exp Eye Res | Authors: Qianwen Bu | Species: human | Application: WB - LIMS2 downregulation is associated with tumor progression and immune landscape alterations in lung adenocarcinoma.
Journal: Discov Oncol | Authors: Huanhuan Wang - PDK4 acts via hippo signaling to inhibit ferroptosis and reduce gemcitabine sensitivity in pancreatic cancer.
Journal: J Mol Histol | Authors: Lei Jin | Species: human | Application: WB - Ruscogenin ameliorates dasatinib-induced intestinal barrier dysfunction via ErbB4/YAP and ROCK/MLC pathways
Journal: J Nat Med | Authors: Juan Huang | Species: mouse,human | Application: WB - Quercetin attenuates cisplatin-induced muscle atrophy via Hippo/YAP1 signaling pathway.
Journal: J Nat Med | Authors: Jingchun Lv - IGF2BP1 enhances the stability of SIK2 mRNA through m6A modification to promote non-small cell lung cancer progression
Journal: Biochem Biophys Res Commun | Authors: Yan Xu | Species: human | Application: WB - Neu-P11 Improves Type 2 Diabetes Mellitus Immune Function by Inhibiting the Hippo Signaling Pathway.
Journal: Int J Endocrinol | Authors: Shichang Cai | Species: mouse,rat | Application: WB - Integrative Phosphoproteomic and Metabolomic Analysis of Disruption of Metabolic Homeostasis in Breast Cancer: A Pilot Study
Journal: Biochem Genet | Authors: Yicong Niu | Species: human | Application: WB - RBM10 suppresses malignant transformation in endometrial cancer via the Hippo-YAP signaling pathway
Journal: Am J Transl Res | Authors: Wei Shi | Species: human | Application: WB - The protective role of quercetin in cataract lenses and its mechanisms related to the hippo signaling pathway.
Journal: Int Ophthalmol | Authors: Sheng Miao | Species: mouse | Application: WB
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