CAPRIN2 Polyclonal antibody 20766-1-AP proteintech
$149.00
In stock
SKU
20766-1-AP
| Catalog Number | 20766-1-AP |
| Synonyms | Gastric cancer multidrug resistance-associated protein, EEG1, Cytoplasmic activation/proliferation-associated protein 2, Caprin-2, C1QDC1 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Applications | WB, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | Y79 cells, SH-SY5Y cells, HEK-293 cells |
| Tested Applications — Positive IP detected in | SH-SY5Y cells |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Positive WB detected in | Y79 cells, SH-SY5Y cells, HEK-293 cells |
| Positive IP detected in | SH-SY5Y cells |
| Positive IF/ICC detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag14745 Product name: Recombinant human CAPRIN2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 970-1128 aa of BC007520 Sequence: MTPVDVPVTNPAATILPVHVYPLPQQMRVAFSAARTSNLAPGTLDQPIVFDLLLNNLGETFDLQLGRFNCPVNGTYVFIFHMLKLAVNVPLYVNLMKNEEVLVSAYANDGAPDHETASNHAILQLFQGDQIWLRLHRGAIYGSSWKYSTFSGYLLYQD Predict reactive species |
| Full Name | caprin family member 2 |
| Calculated Molecular Weight | 1127 aa, 126 kDa |
| Observed Molecular Weight | 126-150 kDa |
| GenBank Accession Number | BC007520 |
| Gene Symbol | CAPRIN2 |
| Gene ID (NCBI) | 65981 |
| RRID | AB_10695763 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6IMN6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for CAPRIN2 antibody 20766-1-AP | Download protocol |
| IHC protocol for CAPRIN2 antibody 20766-1-AP | Download protocol |
| IP protocol for CAPRIN2 antibody 20766-1-AP | Download protocol |
| WB protocol for CAPRIN2 antibody 20766-1-AP | Download protocol |
| rat | IHC Neuroendocrinology Transcriptional and post-transcriptional regulation of oxytocin and vasopressin gene expression by CREB3L1 and CAPRIN2. Authors - Soledad Bárez-López View Article |
| mouse | WB, IF Dev Dyn Deficiency of the RNA binding protein Caprin2 causes lens defects and features of Peters anomaly. Authors - Soma Dash KO Validated View Article |
| Paul (Verified Customer) (04-29-2019) | Antibody works very well for WB and IP from HEK293T cells, and works well for WB and IF from rat tissue samples. However, I couldn't get the antibody to work for IP from rat neuronal samples. Applications: Western Blot, Immunofluorescence, Immunoprecipitation, Primary Antibody Dilution: 1:1000 Cell Tissue Type: HEK293T cells, Rat neuronal samples CAPRIN2 Antibody Western Blot,Immunofluorescence,Immunoprecipitation, validation (1:1000 dilution) in HEK293T cells, Rat neuronal samples (Cat no:20766-1-AP) |
| Criseyda (Verified Customer) (09-21-2018) | Two possibilities: Antibody is not detecting Carping protein well or Caprin2 is not expressed in these cells. Applications: Western Blot, Primary Antibody Dilution: 1:500 Cell Tissue Type: MRC-9 Lung Fiberblasts CAPRIN2 Antibody Western Blot, validation (1:500 dilution) in MRC-9 Lung Fiberblasts (Cat no:20766-1-AP) |
| Citations | 2 |
| Dilutions | WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IF/ICC : 1:20-1:200 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- Transcriptional and post-transcriptional regulation of oxytocin and vasopressin gene expression by CREB3L1 and CAPRIN2.
Journal: Neuroendocrinology | Authors: Soledad Bárez-López | Species: rat | Application: IHC - Deficiency of the RNA binding protein Caprin2 causes lens defects and features of Peters anomaly.
Journal: Dev Dyn | Authors: Soma Dash KO Validated | Species: mouse | Application: WB, IF
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