CARD9 Polyclonal antibody 10669-1-AP proteintech
$149.00
In stock
SKU
10669-1-AP
| Catalog Number | 10669-1-AP |
| Synonyms | Caspase recruitment domain-containing protein 9, hCARD9 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IP, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HL-60 cells, THP-1 cells |
| Tested Applications — Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:3000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | HL-60 cells, THP-1 cells |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag1057 Product name: Recombinant human CARD9 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 193-492 aa of BC008877 Sequence: SEEKGAALMRNRDLQLEIDQLKHSLMKAEDDCKVERKHTLKLRHAMEQRPSQELLWELQQEKALLQARVQELEASVQEGKLDRSSPYIQVLEEDWRQALRDHQEQANTIFSLRKDLRQGEARRLRCMEEKEMFELQCLALRKDSKMYKDRIEAILLQMEEVAIERDQAIATREELHAQHARGLQEKDALRKQVRELGEKADELQLQVFQCEAQLLAVEGRLRRQQLETLVLSSDLEDGSPRRSQELSLPQDLEDTQLSDKGCLAGGGSPKQPFAALHQEQVLRNPHDAGPAGLPGIGAVC Predict reactive species |
| Full Name | caspase recruitment domain family, member 9 |
| Calculated Molecular Weight | 62 kDa |
| Observed Molecular Weight | 55-62 kDa |
| GenBank Accession Number | BC008877 |
| Gene Symbol | CARD9 |
| Gene ID (NCBI) | 64170 |
| RRID | AB_2228323 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H257 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for CARD9 antibody 10669-1-AP | Download protocol |
| WB protocol for CARD9 antibody 10669-1-AP | Download protocol |
| human | WB Plast Reconstr Surg Chronic Candida albicans Meningitis in a 4-Year-Old Girl with a Homozygous Mutation in the CARD9 Gene (Q295X). Authors - Martin Herbst View Article |
| mouse | WB Front Immunol TREM1 Regulates Neuroinflammatory Injury by Modulate Proinflammatory Subtype Transition of Microglia and Formation of Neutrophil Extracellular Traps via Interaction With SYK in Experimental Subarachnoid Hemorrhage. Authors - Xinyan Wu View Article |
| Citations | 12 |
| Dilutions | WB : 1:500-1:3000 IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
CARD9, a member of the caspase recruitment domain (CARD) protein family, plays an important regulatory role in cell apoptosis. Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2) characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CARD9 is highly expressed in spleen and regulate activation of NF-kappa-B by interaction with postive regulator of apoptosis BCL10 via the CARD domain. Protocols Product Specific Protocols IHC protocol for CARD9 antibody 10669-1-AP Download protocol WB protocol for CARD9 antibody 10669-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Invasive fungal infection and impaired neutrophil killing in human CARD9 deficiency.
Journal: Blood | Authors: Drewniak Agata A | Species: human | Application: WB - CARD9-Dependent Neutrophil Recruitment Protects against Fungal Invasion of the Central Nervous System.
Journal: PLoS Pathog | Authors: Rebecca A Drummond | Species: human | Application: WB - Inherited CARD9 Deficiency in a Child with Invasive Disease Due to Exophiala dermatitidis and Two Older but Asymptomatic Siblings.
Journal: J Clin Immunol | Authors: Yusuke Imanaka | Species: human | Application: WB - Inhibition of Dectin-1 Ameliorates Neuroinflammation by Regulating Microglia/Macrophage Phenotype After Intracerebral Hemorrhage in Mice.
Journal: Transl Stroke Res | Authors: Xiongjie Fu | Species: mouse | Application: WB - Chronic Candida albicans Meningitis in a 4-Year-Old Girl with a Homozygous Mutation in the CARD9 Gene (Q295X).
Journal: Plast Reconstr Surg | Authors: Martin Herbst | Species: human | Application: WB - TREM1 Regulates Neuroinflammatory Injury by Modulate Proinflammatory Subtype Transition of Microglia and Formation of Neutrophil Extracellular Traps via Interaction With SYK in Experimental Subarachnoid Hemorrhage.
Journal: Front Immunol | Authors: Xinyan Wu | Species: mouse | Application: WB - Rad50 promotes ovarian cancer progression through NF-κB activation.
Journal: J Cell Mol Med | Authors: Yinuo Li | Species: human | Application: WB,IP - Inhibition of Dectin-1 Ameliorates Neuroinflammation by Regulating Microglia/Macrophage Phenotype After Intracerebral Hemorrhage in Mice.
Journal: Transl Stroke Res | Authors: Xiongjie Fu | Species: mouse | Application: WB - Inherited CARD9 Deficiency Due to a Founder Effect in East Asia
Journal: J Clin Immunol | Authors: Dan Tomomasa | Species: human | Application: WB - Early-Onset Invasive Infection Due to Corynespora cassiicola Associated with Compound Heterozygous CARD9 Mutations in a Colombian Patient.
Journal: J Clin Immunol | Authors: Carlos A Arango-Franco | Species: human | Application: WB - Inherited CARD9 Deficiency in a Child with Invasive Disease Due to Exophiala dermatitidis and Two Older but Asymptomatic Siblings.
Journal: J Clin Immunol | Authors: Yusuke Imanaka | Species: human | Application: WB - Chronic Candida albicans Meningitis in a 4-Year-Old Girl with a Homozygous Mutation in the CARD9 Gene (Q295X).
Journal: Plast Reconstr Surg | Authors: Martin Herbst | Species: human | Application: WB
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