CCDC158 Polyclonal antibody 20396-1-AP proteintech
$149.00
In stock
SKU
20396-1-AP
| Catalog Number | 20396-1-AP |
| Synonyms | Coiled-coil domain-containing protein 158 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, ELISA |
| Applications | WB, IHC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | human testis tissue, mouse testis tissue, rat testis tissue |
| Tested Applications — Positive IP detected in | mouse testis tissue |
| Tested Applications — Positive IHC detected in | human kidney tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:16000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Positive WB detected in | human testis tissue, mouse testis tissue, rat testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human kidney tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag14049 Product name: Recombinant human CCDC158 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-201 aa of BC035224 Sequence: MESKAWESNNEDLLSSSGVTSNGGSSSSFFVSSIRGTIIENTSSAGTLTQVPFFPKYEVELDSPRKIIPSPGKEHFERVLEEYSHQVKDLQRRLNESNELHEKQKFYLRQSVIDLQTKLQEMQMERDAMADIRRRESQSQEDLRNQLQNTVHELEAAKCLKEDMLKDSNTQIEQLRKMMLSHEGVLQEIRSILVDFEEASG Predict reactive species |
| Full Name | coiled-coil domain containing 158 |
| Calculated Molecular Weight | 1113 aa, 127 kDa |
| Observed Molecular Weight | 127-140 kDa |
| GenBank Accession Number | BC035224 |
| Gene Symbol | CCDC158 |
| Gene ID (NCBI) | 339965 |
| RRID | AB_10694278 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5M9N0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for CCDC158 antibody 20396-1-AP | Download protocol |
| IP protocol for CCDC158 antibody 20396-1-AP | Download protocol |
| WB protocol for CCDC158 antibody 20396-1-AP | Download protocol |
| human | IHC,IF J Cell Physiol CCDC158: A novel regulator in renal proximal tubular endocytosis unveiled through exome sequencing and interactome analysis Authors - Tjessa Bondue View Article |
| Citations | 1 |
| Dilutions | WB : 1:2000-1:16000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- CCDC158: A novel regulator in renal proximal tubular endocytosis unveiled through exome sequencing and interactome analysis
Journal: J Cell Physiol | Authors: Tjessa Bondue | Species: human | Application: IHC,IF
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