CCDC22 Polyclonal antibody 16636-1-AP proteintech
$149.00
In stock
SKU
16636-1-AP
| Catalog Number | 16636-1-AP |
| Synonyms | coiled coil domain containing 22, Coiled coil domain containing protein 22, Coiled-coil domain-containing protein 22, CXorf37, JM1 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IP, CoIP, ELISA |
| Applications | WB, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293 cells, HepG2 cells, human brain tissue, mouse colon tissue, mouse lung tissue, rat colon tissue, HeLa cells, Jurkat cells |
| Tested Applications — Positive IP detected in | mouse lung tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:3000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Positive WB detected in | HEK-293 cells, HepG2 cells, human brain tissue, mouse colon tissue, mouse lung tissue, rat colon tissue, HeLa cells, Jurkat cells |
| Positive IP detected in | mouse lung tissue |
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag9980 Product name: Recombinant human CCDC22 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 283-628 aa of BC000972 Sequence: AKTGAPKGSRFTHSEKFTFHLEPQAQATQVSDVPATSRRPEQVTWAAQEQELESLREQLEGVNRSIEEVEADMKTLGVSFVQAESECRHSKLSTAEREQALRLKSRAVELLPDGTANLAKLQLVVENSAQRVIHLAGQWEKHRVPLLAEYRHLRKLQDCRELESSRRLAEIQELHQSVRAAAEEARRKEEVYKQLMSELETLPRDVSRLAYTQRILEIVGNIRKQKEEITKILSDTKELQKEINSLSGKLDRTFAVTDELVFKDAKKDDAVRKAYKYLAALHENCSQLIQTIEDTGTIMREVRDLEEQIETELGKKTLSNLEKIREDYRALRQENAGLLGRVREA Predict reactive species |
| Full Name | coiled-coil domain containing 22 |
| Calculated Molecular Weight | 71 kDa |
| Observed Molecular Weight | 71 kDa |
| GenBank Accession Number | BC000972 |
| Gene Symbol | CCDC22 |
| Gene ID (NCBI) | 28952 |
| RRID | AB_2072065 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60826 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IP protocol for CCDC22 antibody 16636-1-AP | Download protocol |
| WB protocol for CCDC22 antibody 16636-1-AP | Download protocol |
| human | WB Mol Metab Genome-wide CRISPR screen identified a role for commander complex mediated ITGB1 recycling in basal insulin secretion. Authors - Liu Yang View Article |
| mouse | WB Nat Commun CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL. Authors - Paulina Bartuzi View Article |
| Citations | 21 |
| Dilutions | WB : 1:500-1:3000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells.
Journal: Cell | Authors: Zharko Daniloski | Species: human | Application: WB - Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling.
Journal: Nat Cell Biol | Authors: Kerrie E McNally | Species: human | Application: WB - A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.
Journal: Nat Commun | Authors: Yunkai Zhu | Species: human | Application: WB - CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL.
Journal: Nat Commun | Authors: Paulina Bartuzi | Species: mouse | Application: WB - Endosomal sorting of Notch receptors through COMMD9-dependent pathways modulates Notch signaling.
Journal: J Cell Biol | Authors: Haiying Li | Species: human | Application: WB,IP - Genome-wide CRISPR screen identified a role for commander complex mediated ITGB1 recycling in basal insulin secretion.
Journal: Mol Metab | Authors: Liu Yang | Species: human | Application: WB
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