MKS1 Polyclonal antibody 16206-1-AP proteintech
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16206-1-AP
| Catalog Number | 16206-1-AP |
| Synonyms | BBS13, MKS 1, Tectonic-like complex member MKS1 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293 cells, mouse uterus tissue, SH-SY5Y cells, HeLa cells, mouse testis tissue, rat testis tissue |
| Tested Applications — Positive IP detected in | HEK-293 cells |
| Tested Applications — Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF detected in | hTERT-RPE1 cells and Mouse embryonic fibroblasts |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | HEK-293 cells, mouse uterus tissue, SH-SY5Y cells, HeLa cells, mouse testis tissue, rat testis tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | hTERT-RPE1 cells and Mouse embryonic fibroblasts |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag9177 Product name: Recombinant human BBS13 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-321 aa of BC010061 Sequence: MTTAASEVPSFLVERMANVRRRRQDRRGMEGGILKSRIVTWEPSEEFVRNNHVINTPLQTMHIMADLGPYKKLGYKKYEHVLCTLKVDSNGVITVKPDFTGLKGPYRIETEGEKQELWKYTIDNVSPHAQPEEEERERRVFKDLYGRHKEYLSSLVGTDFEMTVPGALRLFVNGEVVSAQGYEYDNLYVHFFVELPTAHWSSPAFQQLSGVTQTCTTKSLAMDKVAHFSYPFTFEAFFLHEDESSDALPEWPVLYCEVLSLDFWQRYRVEGYGAVVLPATPGSHTLTVSTWRPVELGTVAELRRFFIGGSLELEDLSYVRI Predict reactive species |
| Full Name | Meckel syndrome, type 1 |
| Calculated Molecular Weight | 559 aa, 65 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC010061 |
| Gene Symbol | MKS1 |
| Gene ID (NCBI) | 54903 |
| RRID | AB_10637856 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NXB0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for MKS1 antibody 16206-1-AP | Download protocol |
| IHC protocol for MKS1 antibody 16206-1-AP | Download protocol |
| IP protocol for MKS1 antibody 16206-1-AP | Download protocol |
| WB protocol for MKS1 antibody 16206-1-AP | Download protocol |
| mouse,human | WB,IF Nat Genet A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition. Authors - Garcia-Gonzalo Francesc R FR View Article |
| mouse | IF Nat Cell Biol Lineage specificity of primary cilia in the mouse embryo. Authors - Fiona K Bangs View Article |
| human | IF Nat Commun Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis. Authors - Qingwen Xu View Article |
| Citations | 33 |
| Dilutions | WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF : 1:20-1:200 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
MKS1 (Meckel syndrome type 1 protein) is a 559-amino acid protein that contains a conserved B9 domain. It is a component of a large protein complex which localizes to the ciliary transition zone and regulates mammalian ciliogenesis and ciliary membrane composition (PMID: 21725307). MKS1 is required for ciliary structure and function, and is involved in centrosome migration to the apical cell surface during early ciliogenesis (PMID: 17185389; 19515853). Broad tissue expression of the MKS1 gene has been reported (PMID: 16415886). Defects in MKS1 are the cause of Meckel syndrome type 1 (MKS1), an autosomal recessive lethal malformation syndrome characterized by renal cystic dysplasia, central nervous system malformations, and hepatic developmental defects (PMID: 16415886). In addition, defects in MKS1 are also the cause of Bardet-Biedl syndrome type 13 (BBS13) (PMID: 18327255). Protocols Product Specific Protocols IF protocol for MKS1 antibody 16206-1-AP Download protocol IHC protocol for MKS1 antibody 16206-1-AP Download protocol IP protocol for MKS1 antibody 16206-1-AP Download protocol WB protocol for MKS1 antibody 16206-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.
Journal: Nat Genet | Authors: Garcia-Gonzalo Francesc R FR | Species: mouse,human | Application: WB,IF - The spinocerebellar ataxia-associated gene Tau tubulin kinase 2 controls the initiation of ciliogenesis.
Journal: Cell | Authors: Goetz Sarah C SC | Species: mouse | Application: IF - Lineage specificity of primary cilia in the mouse embryo.
Journal: Nat Cell Biol | Authors: Fiona K Bangs | Species: mouse | Application: IF - Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.
Journal: Sci Adv | Authors: Chuan Chen | Species: human | Application: IF - Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.
Journal: Nat Commun | Authors: Hai-Qing Tu | Species: human | Application: IF - Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis.
Journal: Nat Commun | Authors: Qingwen Xu | Species: human | Application: IF - An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.
Journal: Nat Commun | Authors: Karsten Boldt | Species: human | Application: IF - Loss of RPGR disrupts motile cilia and causes primary ciliary dyskinesia by affecting F-actin dynamics.
Journal: J Clin Invest | Authors: Yang Wu | Species: human | Application: IF - Primary cilia formation requires the Leigh syndrome-associated mitochondrial protein NDUFAF2
Journal: J Clin Invest | Authors: Chien-Hui Lo | Species: human | Application: IF - Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.
Journal: Sci Adv | Authors: Chuan Chen | Species: human | Application: IF - ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.
Journal: Dev Cell | Authors: Jie Ran | Species: mouse | Application: WB - A microscopy-based CRISPR screening platform enables organellar functional genomics and illuminates ciliary biology.
Journal: Dev Cell | Authors: Jingbo Sun | Species: mouse,human | Application: IF - Cell type-specific regulation of ciliary transition zone assembly in vertebrates.
Journal: EMBO J | Authors: Antonia Wiegering | Species: mouse | Application: IF - TMEM231, mutated in orofaciodigital and Meckel syndromes, organizes the ciliary transition zone.
Journal: J Cell Biol | Authors: Elle C Roberson - The small GTPase RSG1 controls a final step in primary cilia initiation.
Journal: J Cell Biol | Authors: Stephanie O Agbu | Species: mouse | Application: IF - LUBAC regulates ciliogenesis by promoting CP110 removal from the mother centriole.
Journal: J Cell Biol | Authors: Xiao-Lin Shen | Species: human | Application: IF - INPP5E regulates phosphoinositide-dependent cilia transition zone function.
Journal: J Cell Biol | Authors: Jennifer M Dyson | Species: mouse | Application: IF - Multi-omics analysis delineates molecular signatures of spinal ependymal tumor.
Journal: Cell Oncol (Dordr) | Authors: Weihao Liu | Species: human | Application: IF - EVC-EVC2 complex stability and ciliary targeting are regulated by modification with ubiquitin and SUMO
Journal: Front Cell Dev Biol | Authors: Pablo Barbeito | Species: mouse | Application: IF - Deacetylation of α-tubulin and cortactin is required for HDAC6 to trigger ciliary disassembly.
Journal: Sci Rep | Authors: Jie Ran | Species: human | Application: WB - Superresolution Pattern Recognition Reveals the Architectural Map of the Ciliary Transition Zone.
Journal: Sci Rep | Authors: T Tony Yang | Species: human | Application: IF - Glucose deprivation induces primary cilium formation through mTORC1 inactivation.
Journal: J Cell Sci | Authors: Kengo Takahashi | Species: human | Application: IF - Acute versus chronic loss of mammalian Azi1/Cep131 results in distinct ciliary phenotypes.
Journal: PLoS Genet | Authors: Emma A Hall | Species: mouse | Application: IF - Super-Resolution Imaging Reveals TCTN2 Depletion-Induced IFT88 Lumen Leakage and Ciliary Weakening.
Journal: Biophys J | Authors: Rueyhung Roc Weng | Species: human | Application: IF - MKS1 regulates ciliary INPP5E levels in Joubert syndrome.
Journal: J Med Genet | Authors: Gisela G Slaats | Species: human | Application: WB,IF - Cilia gene mutations cause atrioventricular septal defects by multiple mechanisms.
Journal: Hum Mol Genet | Authors: Ozanna Burnicka-Turek | Species: mouse | Application: WB - Visualizing renal primary cilia.
Journal: Nephrology (Carlton) | Authors: James A Deane | Species: human | Application: IF - Fused (Stk36) is a ciliary protein required for central pair assembly and motile cilia orientation in the mammalian oviduct.
Journal: Dev Dyn | Authors: Nozawa Yoko Inès YI | Species: mouse | Application: IF - Regulation of canonical Wnt signalling by the ciliopathy protein MKS1 and the E2 ubiquitin-conjugating enzyme UBE2E1.
Journal: Elife | Authors: Katarzyna Szymanska | Species: mouse | Application: WB,IF - Two functional forms of the Meckel-Gruber syndrome protein TMEM67 generated by proteolytic cleavage by ADAMTS9 mediate Wnt signaling and ciliogenesis
Journal: bioRxiv | Authors: Manu Ahmed | Application: IF - Regulation of canonical Wnt signalling by the ciliopathy protein MKS1 and the E2 ubiquitin-conjugating enzyme UBE2E1.
Journal: Elife | Authors: Katarzyna Szymanska | Species: mouse | Application: WB,IF - The multifaceted role of EXOC6A in ciliogenesis.
Journal: Elife | Authors: Te-Lin Lin | Species: human | Application: IF - A WDR35-dependent coat protein complex transports ciliary membrane cargo vesicles to cilia.
Journal: Elife | Authors: Tooba Quidwai | Species: mouse | Application: IF
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