CCS Monoclonal antibody 68341-1-Ig proteintech
$149.00
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68341-1-Ig
| Catalog Number | 68341-1-Ig |
| Synonyms | CCS |
| Host | Mouse |
| Reactivity | Human, mouse, rat |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Mouse / IgG2a |
| Tested Applications — Positive WB detected in | LNCaP cells, HepG2 cells, A549 cells |
| Tested Applications — Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Positive WB detected in | LNCaP cells, HepG2 cells, A549 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Tested Reactivity | Human, mouse, rat |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag18860 Product name: Recombinant human CCS protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-274 aa of BC105016 Sequence: MASDSGNQGTLCTLEFAVQMTCQSCVDAVRKSLQGVAGVQDVEVHLEDQMVLVHTTLPSQEVQALLEGTGRQAVLKGMGSGQLQNLGAAVAILGGPGTVQGVVRFLQLTPERCLIEGTIDGLEPGLHGLHVHQYGDLTNNCNSCGNHFNPDGASHGGPQDSDRHRGDLGNVRADADGRAIFRMEDEQLKVWDVIGRSLIIDEGEDDLGRGGHPLSKITGNSGERLACGIIARSAGLFQNPKQICSCDGLTIWEERGRPIAGKGRKESAQPPAHL Predict reactive species |
| Full Name | copper chaperone for superoxide dismutase |
| Calculated Molecular Weight | 274 aa, 29 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC105016 |
| Gene Symbol | CCS |
| Gene ID (NCBI) | 9973 |
| RRID | AB_3085065 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | O14618 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for CCS antibody 68341-1-Ig | Download protocol |
| IHC protocol for CCS antibody 68341-1-Ig | Download protocol |
| WB protocol for CCS antibody 68341-1-Ig | Download protocol |
| Citations | 1 |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:50-1:500 IF/ICC : 1:500-1:2000 |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Clone Number | 3F5E5 |
| Conjugation | Unconjugated |
Copper chaperone for superoxide dismutase (CCS) is a soluble factor that converts copper into copper-zinc superoxide dismutase ( SOD1 ). CCS and SOD1 interact directly in vivo and in vitro, and this interaction is mediated by homologous domains in each protein. CCS not only interacts with wild-type SOD1 but also interacts with SOD1 containing common missense mutations, resulting in FALS. Protocols Product Specific Protocols IF protocol for CCS antibody 68341-1-Ig Download protocol IHC protocol for CCS antibody 68341-1-Ig Download protocol WB protocol for CCS antibody 68341-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Xuefu Zhuyu Decoction Reduces Excessive Collagen Deposition and Crosslinking in Myocardial Fibrosis via CTR1-Copper-LOX Axis.
Journal: Chin J Integr Med | Authors: Ya-Fen Wang
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